Most biosensing techniques require complex processing steps that generate prolonged workflows and introduce potential points of error. Here, we report an acoustic pipette to purify and label biomarkers in 70 minutes. A key aspect of this technology is the use of functional negative acoustic contrast particles (fNACPs), which display biorecognition motifs for the specific capture of biomarkers from whole blood. Because of their large size and compressibility, the fNACPs robustly trap along the pressure antinodes of a standing wave and separate from blood components in under 60 seconds with >99% efficiency. fNACPs are subsequently fluorescently labeled in the pipette and are analyzed by both a custom, portable fluorimeter and flow cytometer. We demonstrate the detection of anti-ovalbumin antibodies from blood at picomolar levels (35 to 60 pM) with integrated controls showing minimal nonspecific adsorption. Overall, this system offers a simple and versatile approach for the rapid, sensitive, and specific capture of biomolecules.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482303 | PMC |
| http://dx.doi.org/10.1126/sciadv.ado9018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acoustic pipette and biofunctional elastomeric microparticle system for rapid picomolar-level biomolecule detection in whole blood.
Sci Adv
October 2024
Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80303, USA.
Most biosensing techniques require complex processing steps that generate prolonged workflows and introduce potential points of error. Here, we report an acoustic pipette to purify and label biomarkers in 70 minutes. A key aspect of this technology is the use of functional negative acoustic contrast particles (fNACPs), which display biorecognition motifs for the specific capture of biomarkers from whole blood.
View Article and Find Full Text PDFImmediate reuse of patch-clamp pipettes after ultrasonic cleaning.
Sci Rep
January 2024
Systems Neurophysiology, Institute of Biology II, RWTH-Aachen University, Aachen, Germany.
The patch-clamp technique has revolutionized neurophysiology by allowing to study single neuronal excitability, synaptic connectivity, morphology, and the transcriptomic profile. However, the throughput in recordings is limited because of the manual replacement of patch-pipettes after each attempt which are often also unsuccessful. This has been overcome by automated cleaning the tips in detergent solutions, allowing to reuse the pipette for further recordings.
View Article and Find Full Text PDFAn introductory laboratory class in sonochemistry.
Ultrason Sonochem
December 2023
Warsaw University of Technology, Faculty of Chemistry, Noakowski Street 3, 00-664 Warsaw, Poland. Electronic address:
This article proposes a substantive scope and scenario of a laboratory class that introduces students to the field of sonochemistry. The class requires only basic laboratory equipment - typical laboratory glassware like graduated pipettes and conical flasks, as well as simple inorganic chemicals. It is designed to acquaint students with fundamental aspects of sonochemistry.
View Article and Find Full Text PDFElevation of Intra-Cellular Calcium in Nucleus Pulposus Cells with Micro-Pipette-Guided Ultrasound.
Ultrasound Med Biol
July 2021
Department of Biomedical Engineering, National Taiwan University, Taipei, Taiwan. Electronic address:
Modulation of intra-cellular calcium by ultrasound offers a possible means for therapeutic applications. One such possibility is the modulation of nucleus pulposus cells as a preventive measure for inter-vertebral disc degeneration. We report a cellular stimulation device (micro-pipette ultrasound) using a glass micro-pipette as a waveguide to deliver ultrasound through the pipette tip and to elevate intra-cellular calcium in nucleus pulposus cells.
View Article and Find Full Text PDFRing-Focusing Fresnel Acoustic Lens for Long Depth-of-Focus Focused Ultrasound with Multiple Trapping Zones.
J Microelectromech Syst
October 2020
Department of Electrical and Computer Engineering, University of Southern California, Los Angeles, CA 90089-0271, USA.
This paper describes a novel acoustic transducer with dual functionality based on 1-mm-thick lead zirconate titanate (PZT) substrate with a modified air-cavity Fresnel acoustic lens on top. Designed to let ultrasound waves focus over an annular ring region, the lens generates a long depth-of-focus Bessel-like focal beam and multiple trapping zones based on quasi-Airy beams and bottle beams. With 2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!