AI Article Synopsis
- * The identification and antibiotic susceptibility testing were conducted using advanced techniques, including MALDI-TOF MS and broth microdilution methods, revealing resistance to multiple antibiotics.
- * Whole-genome sequencing and conjugation experiments showed that the bla gene is located on a mobile plasmid, which raises concerns about the potential spread of antibiotic resistance, indicating the need for increased surveillance in public health.
Article Abstract
We report the isolation of a bla-positive, tigecycline-resistant Klebsiella aerogenes strain KA04 from a Chinese inpatient's fecal sample. Species identification was performed using MALDI-TOF MS. The antibiotic susceptibilities were assessed via the broth microdilution method. To elucidate the transmission and genetic structure of the bla gene, conjugation assays and whole-genome sequencing (WGS) were performed. KA04 displayed resistance to carbapenems, quinolones, piperacillin/tazobactam and tigecycline. Through WGS and conjugation experiments, it was possible to confirm bla and qnrS1 genes causing antibiotic resistance were located on a 51-kb IncX3 type mobile plasmid, bla gene could be successfully transferred into E. coli EC600 at a conjugation frequency of 1.1 × 10. tet(A) gene was located on both the chromosome and non-transmissible IncFIB(K) plasmid. This is a tigecycline-resistant K. aerogenes harboring bla isolate from human fecal sample, highlighting a significant public health concern. Further comprehensive surveillance is needed.
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