AI Article Synopsis
- Apoptin is a promising anticancer protein that selectively induces apoptosis in tumor cells, particularly in breast cancer lines, highlighting its potential for cancer therapy.
- Researchers successfully created two forms of apoptin fusion proteins (SUMO-PTD4-Apoptin and PTD4-Apoptin) and tested their effects on breast cancer cells (MDA-MB-231 and MCF-7) and normal cells (MCF-10A) using various assays.
- Results showed that SUMO-PTD4-Apoptin demonstrated a lower IC50 value in cancer cells than in normal cells, indicating higher cytotoxicity, while PTD4-Apoptin had similar effects across both cancer
Article Abstract
Backgrounds: Apoptin can induce tumor cell-specific apoptosis in a broad range of human tumor cells and is a potential anticancer therapeutic candidate to kill tumor cells.
Materials And Methods: We designed two structures of apoptin fusion protein, SUMO-PTD4-Apoptin, and PTD4-Apoptin. To express these fusion proteins, BL21(DE3) was employed. MTT assay, Flow cytometry, and cell cycle analysis were used to investigate the function of proteins on two breast cancer cell lines (MDA-MB-231 and MCF-7) and MCF 10A cell line (as normal cells).
Results: Expression of the recombinant SUMO-PTD4-Apoptin and PTD4-Apoptin in BL21(DE3) was successful. MTT assay results showed that the IC50 was 6.4 µg/ml for SUMO-PTD4-Apoptin in MDA-MB-231 and was 9.3 after 24 h of treatment in MCF-7. The specific cytotoxicity in both cell lines is significant in comparison with MCF-10A, which is used as a normal cell line (IC50 = 29.4). The IC50 for PTD4-Apoptin was 11.07 µg/ml after 24 h of treatment in MDA-MB-231, while the IC50 of PTD4-Apoptin for MCF7 cells was not significantly different from normal cells. The flow cytometry analysis displayed a significant increment in the apoptosis and late apoptosis number in the MDA-MB-231 cells after treatment with SUMO-PTD4-Apoptin and PTD4-Apoptin protein. PTD4-Apoptin and SUMO-PTD4-Apoptin treatment of MDA-MB-231 cells caused a noteworthy increase in the G0-G1 phase and a reduction in the cell population of S and M/G2.
Conclusion: This study demonstrates that the fusion of PTD4-Apoptin to SUMO-PTD4-Apoptin could provide an effective method to help enhance the expression and solubility of heterologous Apoptin in BL21 (DE3).
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11478704 | PMC |
| http://dx.doi.org/10.4103/abr.abr_295_23 | DOI Listing |
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