AI Article Synopsis
- - The study investigates the relationship between EasyNAT system time threshold (Tt) values and Cobas z480 cycle threshold (Ct) values in detecting SARS-CoV-2 from oropharyngeal swabs, using retrospective data from a clinical lab.
- - A total of 277 EasyNAT-positive samples showed a 100% concordance with Cobas z480, indicating that as Tt values increased, the mean and maximum Ct values for specific viral genes also increased.
- - The findings suggest that the EasyNAT system is a reliable, efficient alternative for SARS-CoV-2 detection in hospitals, particularly in facilities lacking advanced PCR technology, with Tt values of ≤6 serving as a reference for
Article Abstract
Objectives: This study aimed to evaluate the potential relationship between the time threshold (Tt) values of a commercial EasyNAT system, which is based on cross priming amplification (CPA) technology, and the cycle threshold (Ct) values of the Cobas z480 analyzer, which is based on a real-time fluorescence polymerase chain reaction (PCR) method, in the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) from oropharyngeal swabs. Design and Methods: Data were retrospectively collected from a clinical laboratory between December 4, 2022 and July 1, 2024.
Results: A total of 277 EasyNAT-positive samples (Tt values from 3.83 to 29.5) were simultaneously investigated using the Cobas z480 analyzer (Ct values from 10.74 to 38.78). The concordance rate between the two systems was 100 %. Among the positive samples, the mean and maximum PCR Ct values of O and N genes increased in line with increasing Tt values of the left and right amplification areas of the EasyNAT system. The maximum Ct values of the O or N gene determined by the Cobas z480 analyzer were no more than 29.52 when the Tt values of the left or right amplification areas of the UC0116 analyzer were no more than 6.
Conclusions: The safe, simple, fast, accurate, and automatic EasyNAT system used in conjunction with a PCR system might be a better choice for the detection of SARS-CoV-2 in hospitals, especially in settings without sophisticated PCR facilities. The Tt value (≤6) of the EasyNAT system can be a reference index for estimating the maximum Ct value (29.52) in SARS-CoV-2-positive samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11474176 | PMC |
| http://dx.doi.org/10.1016/j.plabm.2024.e00431 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Clinical value of automated EasyNAT system for the diagnosis of tuberculosis in paraffin-embedded tissues].
Beijing Da Xue Xue Bao Yi Xue Ban
December 2024
Department of Pathology, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Objective: Assessing the accuracy of automated EasyNAT system for rapidly detecting paraffin-embedded tissue for the diagnosis of tuberculosis.
Methods: A retrospective analysis was conducted on 134 patients, comprising 101 with confirmed tuberculosis and 33 without tuberculosis, treated at Beijing Chest Hospital, Capital Medical University, between 2018 and 2022.The clinical diagnostic results served as the standard for assessing the diagnostic performance of the EasyNAT system in comparison to quantitative real-time polymerase chain reaction (qPCR) for tuberculosis detection in paraffin-embedded tissues.
Establishment of cross-priming amplification for point-of-care detection of and non-tuberculosis mycobacteria.
Heliyon
October 2024
Department of Clinical Laboratory, Longyan First Affiliated Hospital of Fujian Medical University, Longyan, Fujian, China.
Article Synopsis
- - The study aims to simplify sputum sample preparation for point-of-care testing (POCT) of MTB and non-tuberculosis mycobacteria (NTM) using Cross-Priming Amplification (CPA) technology, particularly in grassroots settings in China.
- - High-concentration guanidine thiocyanate (GTC) was found effective in liquefying sputum and inactivating MTB, with the new CPA method showing strong agreement with traditional RT-PCR testing results.
- - The CPA method demonstrates good accuracy in distinguishing between MTB and NTM, suggesting it is a rapid, simple, and effective alternative for testing, eliminating the need for specialized laboratory facilities.
The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2.
Pract Lab Med
November 2024
Department of Clinical Laboratory, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Article Synopsis
- - The study investigates the relationship between EasyNAT system time threshold (Tt) values and Cobas z480 cycle threshold (Ct) values in detecting SARS-CoV-2 from oropharyngeal swabs, using retrospective data from a clinical lab.
- - A total of 277 EasyNAT-positive samples showed a 100% concordance with Cobas z480, indicating that as Tt values increased, the mean and maximum Ct values for specific viral genes also increased.
- - The findings suggest that the EasyNAT system is a reliable, efficient alternative for SARS-CoV-2 detection in hospitals, particularly in facilities lacking advanced PCR technology, with Tt values of ≤6 serving as a reference for
Background: This study aimed to effectively evaluate the diagnostic performance of the EasyNAT Mycobacterium tuberculosis complex (MTC) assay for tuberculosis (TB) detection from sputum.
Methods: The retrospectively analyzed data was collected from September 1, 2021, to November 1, 2023, in our hospital.
Results: Forty EasyNAT-positive sputum specimens were simultaneously detected using the GeneXpert MTB/ rifampicin (RIF) assay.
Accurate detection of influenza A virus by use of a novel cross-priming isothermal amplification-based point-of-care assay.
Microbiol Spectr
March 2024
Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Influenza virus is known to cause respiratory tract infections of varying severity in individuals of all ages. The EasyNAT Rapid Flu assay is a newly developed diagnostic test that employs cross-priming isothermal amplification (CPA) to detect and differentiate influenza A and B viruses in human nasopharyngeal (NP) swabs. The aim of this study is to determine the performance characteristics of the EasyNAT Rapid Flu assay for rapid detection of influenza virus.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!