Evaluation of the Eazyplex ID LAMP Assay for the Rapid Diagnosis of Positive Blood Cultures.

Rapid molecular assays can be used to identify pathogens directly from positive blood cultures (BCs) in a timely manner compared to standard methods using subcultures. In this study, the eazyplex ID assay, which is based on loop-mediated amplification (LAMP) and is currently for research use only, was evaluated for the identification of the most common fungal species. A total of 190 BCs were analysed. Sensitivity and specificity were 93.88% and 99.26% for , 89.13% and 100% for (), 100% and 100% for (), 100% and 100% for and 100% and 99.44% for . Sample preparation took approximately 11 min and positive amplification results were obtained between 8.5 and 19 min. The eazyplex ID LAMP assay is an easy-to-use diagnostic tool that can optimise the management of patients with candidemia.