AI Article Synopsis

  • The IA polymorphism is linked to both the Ankyrin Repeat and Kinase Domain-containing I gene (ANKK1) and the D2 dopamine receptor gene, posing challenges in understanding its role in addictions and psychiatric disorders due to unclear ANKK1 functions.
  • Research using SH-SY5Y neuroblastoma models shows that ANKK1 interacts with FARP1, a synapse protein, contributing to neuronal maturation and the activation of the Wnt/PCP pathway while promoting neuritogenesis.
  • Interactions between ANKK1 and WGEF are crucial for regulating RhoGTPases during neuronal differentiation, indicating that ANKK1 may play a significant role in organizing the brain's structural response related to IA-associated

Article Abstract

The IA polymorphism is a marker of both the Ankyrin Repeat and Kinase Domain containing I gene () encoding a RIP-kinase, and the gene for the dopamine receptor D2. Despite a large number of studies of IA in addictions and other psychiatric disorders, there is difficulty in interpreting this genetic phenomenon due to the lack of knowledge about ANKK1 function. In SH-SY5Y neuroblastoma models, we show that ANKK1 interacts with the synapse protein FERM ARH/RhoGEF and Pleckstrin Domain 1 (FARP1), which is a guanine nucleotide exchange factor (GEF) of the RhoGTPases RAC1 and RhoA. ANKK1-FARP1 colocalized in F-ACTIN-rich structures for neuronal maturation and migration, and both proteins activate the Wnt/PCP pathway. ANKK1, but not FARP1, promotes neuritogenesis, and both proteins are involved in neuritic spine outgrowth. Notably, the knockdown of or affects RhoGTPases expression and neural differentiation. Additionally, ANKK1 binds WGEF, another GEF of Wnt/PCP, regulating its interaction with RhoA. During neuronal differentiation, ANKK1-WGEF interaction is downregulated, while ANKK1-FARP1 interaction is increased, suggesting that ANKK1 recruits Wnt/PCP components for bidirectional control of F-ACTIN assembly. Our results suggest a brain structural basis in IA-associated phenotypes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11477271PMC
http://dx.doi.org/10.3390/ijms251910705DOI Listing

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