AI Article Synopsis
- Induced pluripotent stem cell-derived mesenchymal stromal cells (iMSCs) and their extracellular vesicles (iMSC-EVs) are being evaluated as alternatives to traditional primary mesenchymal stromal cells (hUCMSCs) for use in advanced therapies.
- The study found that iMSCs effectively regulate immune responses, showing similar abilities to hUCMSCs in controlling lymphocyte growth and promoting an anti-inflammatory environment.
- Furthermore, iMSC-EVs demonstrated both immunomodulatory and regenerative properties, with enhanced effects observed when iMSCs were treated with pro-inflammatory cytokines, highlighting their potential as therapeutic options.
Article Abstract
Induced pluripotent stem cell (iPSC)-derived mesenchymal stromal cells (iMSCs) offer a promising alternative to primary mesenchymal stromal cells (MSCs) and their derivatives, particularly extracellular vesicles (EVs), for use in advanced therapy medicinal products. In this study we evaluated the immunomodulatory and regenerative potential of iMSCs as well as iMSC-EVs, alongside primary human umbilical cord-derived mesenchymal stromal cells (hUCMSCs). Our findings demonstrate that iMSCs exhibit comparable abilities to hUCMSCs in regulating lymphocyte proliferation and inducing an anti-inflammatory phenotype in monocytes. We also observed decreased TNFα levels and increased IL-10 induction, indicating a potential mechanism for their immunomodulatory effects. Furthermore, iMSC-EVs also showed effective immunomodulation by inhibiting T cell proliferation and inducing macrophage polarization similar to their parental cells. Additionally, iMSC-EVs exhibited pro-regenerative potential akin to hUCMSC-EVs in in vitro scratch assays. Notably, priming iMSCs with pro-inflammatory cytokines significantly enhanced the immunomodulatory potential of iMSC-EVs. These results underscore the considerable promise of iMSCs and iMSCs-EVs as an alternate source for MSC-derived therapeutics, given their potent immunomodulatory and regenerative properties.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11480492 | PMC |
| http://dx.doi.org/10.1038/s41598-024-75956-3 | DOI Listing |
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