Polydopamine-modified 3D flower-like ZnMoO integrated MXene-based label-free electrochemical immunosensor for the food-borne pathogen Listeria monocytogenes detection in milk and seafood.

Talanta

Department of Chemical Engineering & Biotechnology, National Taipei University of Technology, Taipei, 10608, Taiwan; High-value Biomaterials Research and Commercialization Center, National Taipei University of Technology (Taipei Tech), Taipei, 10608, Taiwan. Electronic address:

Published: January 2025

AI Article Synopsis

  • Listeria monocytogenes is a harmful bacterium responsible for listeriosis, often found in ready-to-eat food, making its detection crucial for food safety.
  • The study introduces an innovative, label-free electrochemical immunosensor using a polydopamine-modified zinc molybdate/MXene composite for rapid and effective detection of L. monocytogenes in various food products like milk and seafood.
  • The immunosensor has shown high sensitivity with a low detection limit, good selectivity against other microbes, and impressive stability, suggesting its potential for broader applications in monitoring food safety against multiple pathogens.

Article Abstract

Listeria monocytogenes is a gram-positive bacterium that causes listeriosis in humans. This contaminates the ready-to-eat food products and compromises their safety. Thus, detecting its presence in food samples with high sensitivity and reliability is necessary. Herein, we propose a label-free electrochemical immunosensor based on a mussel-inspired polydopamine-modified zinc molybdate/MXene (PDA@ZnMoO/MXene) composite for effective and rapid detection of L. monocytogenes in food products. Spectrophotometry approaches were employed to examine the resulting composites. Voltammetry and impedimetry techniques were used to confirm the step-by-step assembly of the immunosensor and its sensitive detection of L. monocytogenes in various food products, such as milk and smoked seafood. The results demonstrated the practicality of the constructed immunosensor, with an appreciable linearity of 10-10 CFU/ml and a reasonably low detection limit (LOD, 12 CFU/ml). Moreover, the immunosensor exhibited excellent selectivity for microbial cocktails and acceptable repeatability, reproducibility, and storage stability. Thus, we believe that the proposed sensitive, reliable, and label-free immunosensor based on the PDA surface modification technique for detecting L. monocytogenes can be extended to monitor various food-borne pathogens to ensure food safety.

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http://dx.doi.org/10.1016/j.talanta.2024.127008DOI Listing

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