Listeria monocytogenes is a gram-positive bacterium that causes listeriosis in humans. This contaminates the ready-to-eat food products and compromises their safety. Thus, detecting its presence in food samples with high sensitivity and reliability is necessary. Herein, we propose a label-free electrochemical immunosensor based on a mussel-inspired polydopamine-modified zinc molybdate/MXene (PDA@ZnMoO/MXene) composite for effective and rapid detection of L. monocytogenes in food products. Spectrophotometry approaches were employed to examine the resulting composites. Voltammetry and impedimetry techniques were used to confirm the step-by-step assembly of the immunosensor and its sensitive detection of L. monocytogenes in various food products, such as milk and smoked seafood. The results demonstrated the practicality of the constructed immunosensor, with an appreciable linearity of 10-10 CFU/ml and a reasonably low detection limit (LOD, 12 CFU/ml). Moreover, the immunosensor exhibited excellent selectivity for microbial cocktails and acceptable repeatability, reproducibility, and storage stability. Thus, we believe that the proposed sensitive, reliable, and label-free immunosensor based on the PDA surface modification technique for detecting L. monocytogenes can be extended to monitor various food-borne pathogens to ensure food safety.
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http://dx.doi.org/10.1016/j.talanta.2024.127008 | DOI Listing |
Sci Data
December 2024
Unit of Biostatistics, Epidemiology, and Public Health, Department of Cardiac Thoracic Vascular Sciences and Public Health, University of Padova, via Loredan 18, Padova, 35131, Italy.
This study presents a method for automating the retrieval of key identifies and links to toxicological data from the Joint FAO/WHO Expert Committee on Food Additives (JECFA) database using web scraping techniques. Although the method primarily serves as an automated indexing tool, facilitating organization and access to relevant reports, monographs, and specifications, it significantly enhances the efficiency of navigating the extensive JECFA database. Researchers can then perform more targeted and efficient searches, although additional manual steps are required to extract and structure the detailed toxicological data.
View Article and Find Full Text PDFBMC Nutr
December 2024
Department of Food Science and Postharvest Technology, Faculty of Agriculture and Environment, Gulu University, P.O. Box 166, Gulu, Uganda.
Background: Globally, iron deficiency anaemia is a widespread public health problem affecting vulnerable populations including adolescents. However, over the years, the Uganda Demographic Health Surveys mostly report the status of anaemia for women of reproductive age (15-49 years) and children up to 5 years, leaving out the focus on adolescents. Moreover, high prevalence of anaemia among children below five years could suggest that anaemia still persists at adolescence.
View Article and Find Full Text PDFBMJ Open
December 2024
Laboratory of Human Milk and Lactation Research, Department of Medical Biology, University of Warsaw, Warsaw, Poland.
Introduction: Donor human milk (DHM) is the first alternative if mother's own milk is unavailable or contraindicated. Much DHM research has focused on its nutritional, immunological and biochemical composition in response to various maternal variables, standard human milk banking procedures and storage protocols. The current systematic review protocol, however, aims to systematically gather and analyse existing data pertaining to the impact of these aforementioned factors on the clinical, health-related and developmental outcomes observed in infants fed with DHM.
View Article and Find Full Text PDFBiotechnol Adv
December 2024
Department of Food Science and Biotechnology, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-Gu, Seoul 03760, Republic of Korea.
The selective oxyfunctionalization of unsaturated fatty acids is difficult in chemical reactions, whereas regio- and stereoselective oxyfunctionalization is often performed in biocatalytic synthesis. Fatty acid oxygenases, including hydratases, lipoxygenases, dioxygenases, diol synthases, cytochrome P450 monooxygenases, peroxygenases, and 12-hydroxylases, are used to convert C16 and C18 unsaturated fatty acids to diverse regio- and stereoselective mono-, di-, and trihydroxy fatty acids via selective oxyfunctionalization. The formed hydroxy fatty acids or hydroperoxy fatty acids are metabolized to industrially important oxygenated chemicals such as lactones, green leaf volatiles, and bioplastic monomers, including ω-hydroxy fatty acids, α,ω-dicarboxylic acids, and fatty alcohols, by biocatalysts.
View Article and Find Full Text PDFJ Nutr
December 2024
Nottingham NIHR Biomedical Research Centre and Nottingham Digestive Disease Centre, School of Medicine, University of Nottingham, Nottingham, United Kingdom. Electronic address:
Background: Recent studies show that the increase in breath hydrogen (BH) and symptoms after ingestion of inulin is reduced by co-administering psyllium.
Objectives: To determine if slowing delivery of inulin to the colon by administering it in divided doses would mimic the effect of psyllium. Primary endpoint was the BH area under the curve AUC.
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