spores are produced inside the cytosol of a mother cell. Spore surface assembly requires the SpoVK protein in the mother cell, but its function is unknown. Here, we report that SpoVK is a sporulation-specific, forespore-localized putative chaperone from a distinct higher-order clade of AAA+ ATPases that promotes the peptidoglycan glycosyltransferase activity of MurG during sporulation, even though MurG does not normally require activation during vegetative growth. MurG redeploys to the forespore surface during sporulation, where we show that the local pH is reduced and propose that this change in cytosolic nanoenvironment abrogates MurG function. Further, we show that SpoVK participates in a developmental checkpoint in which improper spore surface assembly mis-localizes SpoVK, which leads to sporulation arrest. The AAA+ ATPase clade containing SpoVK includes specialized chaperones involved in secretion, cell envelope biosynthesis, and carbohydrate metabolism, suggesting that such fine-tuning might be a widespread feature of different subcellular nanoenvironments.
To construct a recombinant strain expressing SpaA and CbpB of for oral administration, we constructed the recombinant plasmid pDG1730-CBJA by fusion PCR and seamless cloning. The plasmid was introduced into . KC strain by natural transformation, and the recombinant strain KC-- was screened out on the plate containing spectinomycin () and confirmed by PCR and starch degradation test.
National Engineering Research Center for Agro-Ecological Big Data Analysis & Application, Anhui University, Hefei, 230601, Anhui, China; School of Electronics and Information Engineering, Anhui University, Hefei, 230601, Anhui, China. Electronic address:
Determination of Fusarium spores is essential for precision control of Fusarium head blight and ensuring agri-food safety. As a highly sensitive real-time detection technique with rich fingerprint information and little influence from water, surface-enhanced Raman spectroscopy (SERS) has been widely applied in the determination of microorganisms. However, fungi determination faces significant challenges including low sensitivity, and poor specificity.
Objective: () is one of the most common causes of healthcare-associated infections (HAIs). Elimination of spores is difficult as they are resistant to common hospital-grade disinfectants. Copper-impregnated surfaces provide continuous reduction of multiple pathogens, potentially lowering the risk of infections.
Department of Food Science, Faculty of AgriSciences, Stellenbosch University, Stellenbosch University., Matieland, South Africa; AgriFood BioSystems and Technovation Research Group, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Stellenbosch University., Matieland, South Africa. Electronic address:
This study focused on the application of micro-nano bubbles (MNB) water generated using air or oxygen (O), as an alternative to chlorine-based wash for fruits. For the in vitro and in vivo investigation, 10 spore or conidia/mL Colletotrichum gloeosporioides suspension was used, and treated with solutions of air- or O-MNB for 30- or 60-min, sodium hypochlorite (NaOCl), and untreated (as control). In the second experiment, freshly harvested guava fruits were washed with tap water (control), NaOCl (standard practice), air- or O-MNB (for 15- or 30-min).
College of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, China.
Lily bulb rot disease has harmed edible lily in recent years, resulting in yield losses in China. As a results, both morphological and molecular techniques must be used to confirm the etiology of storage bulb rot disease on lily bulbs. Lily bulbs with indications of rot symptoms during storage were gathered in Lanzhou, Gansu Province, China.
