AI Article Synopsis
- The study focused on phospholipase D genes in perennial ryegrass, identifying 12 PLD genes, with LpPLDδ3 being a key player in stress responses.
- LpPLDδ3 expression was negatively affected by ABA treatment but positively induced by drought and heat stress, leading to improved stress tolerance when overexpressed in Arabidopsis.
- The overexpression of LpPLDδ3 not only enhanced plant survival rates during stress but also activated heat stress-related genes, revealing a regulatory relationship between LpABF2, LpABF4, and LpPLDδ3.
Article Abstract
The phospholipase Ds (PLDs) are crucial for cellular signalling and play roles in plant abiotic stress response. In this study, we identified 12 PLD genes from the genome data of perennial ryegrass (Lolium perenne), which is widely used as forage and turfgrass. Among them, LpPLDδ3 was significantly repressed by ABA treatment, and induced by drought stress and heat stress treatments. The ectopic overexpression (OE) of LpPLDδ3 in Arabidopsis enhanced plant tolerance to osmotic and heat stress as demonstrated by an increased survival rate and reduced malondialdehyde (MDA) accumulation and electrolyte leakage (EL). Arabidopsis endogenous ABA RESPONSIVE ELEMENT BINDING FACTORs (ABFs) and heat stress responsive genes were elevated in LpPLDδ3 OE lines under osmotic and heat stress treatments. Additionally, overexpression of LpPLDδ3 in perennial ryegrass protoplasts could increase heat stress tolerance and elevate expression level of heat stress responsive genes. Moreover, LpABF2 and LpABF4 depressed the LpPLDδ3 expression by directly binding to its ABRE core-binding motif of promoter region. In summary, LpPLDδ3 was repressed by LpABF2 and LpABF4 and positively involved in perennial ryegrass osmotic and heat stress responses.
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| http://dx.doi.org/10.1111/pce.15211 | DOI Listing |
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