The vastly spreading COVID-19 pneumonia is caused by SARS-CoV-2. Lymphopenia and cytokine levels are tightly associated with disease severity. However, virus-induced immune dysregulation at cellular and molecular levels remains largely undefined. Here, the leukocytes in the pleural effusion, sputum, and peripheral blood biopsies from severe and mild patients were analyzed at single-cell resolution. Drastic T cell hyperactivation accompanying elevated T cell exhaustion was observed, predominantly in pleural effusion. The mechanistic investigation identified a group of CD14 monocytes and macrophages highly expressing and in the biopsies from severe patients, suggesting M2 macrophage polarization. These M2-like cells exhibited up-regulated (M-CSF), and signaling pathways. Further, cell type specific dysregulation of transposable elements was observed in Severe COVID-19 patients. Together, our results suggest that severe SARS-CoV-2 infection causes immune dysregulation by inducing M2 polarization and subsequent T cell exhaustion. This study improves our understanding of COVID-19 pathogenesis.
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http://dx.doi.org/10.1016/j.heliyon.2024.e38688 | DOI Listing |
J Genet Genomics
December 2024
Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Key Lab of Plant factory for Plant Factory Generation-Adding Breeding of Ministry of Agriculture and Rural Affairs, the Advanced Seed Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China; Hainan Institute of Zhejiang University, Sanya, Hainan 572025, China. Electronic address:
Plant Mol Biol
December 2024
Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA.
Heat stress affects various components of photosynthetic machinery of which Rubisco activation inhibition due to heat sensitive Rubisco activase (RCA) is the most prominent. Detailed comparison of RCA coding genes identified a tandem duplication event in the grass family lineage where the duplicated genes showed very different evolutionary pattern. One of the two genes showed high level of sequence conservation whereas the second copy, although present only 1.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
December 2024
National Key Laboratory of Biobased Transportation Fuel Technology, Ocean College, Zhejiang University, Hangzhou, 310027, China.
The halotolerant yeast Scheffersomyces spartinae, commonly found in marine environments, holds significant potential for various industrial applications. Despite this, its genetic characteristics have been relatively underexplored. In this study, we isolated a strain of S.
View Article and Find Full Text PDFBioinform Adv
November 2024
Department of Genomic Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Summary: Transposable elements (TEs), commonly referred to as "mobile elements," constitute DNA segments capable of relocating within a genome. Initially disregarded as "junk DNA" devoid of specific functionality, it has become evident that TEs have diverse influences on an organism's biology and health. The impact of these elements varies according to their location, classification, and their effects on specific genes or regulatory components.
View Article and Find Full Text PDFGenome Biol
December 2024
Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, 1210, Vienna, Austria.
We present GenomeDelta, a novel tool for identifying sample-specific sequences, such as recent transposable element (TE) invasions, without requiring a repeat library. GenomeDelta compares high-quality assemblies with short-read data to detect sequences absent from the short reads. It is applicable to both model and non-model organisms and can identify recent TE invasions, spatially heterogeneous sequences, viral insertions, and hotizontal gene transfers.
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