Fig. 1: Schematic illustration of the principle of the Dual targets driven SPEXPAR assisted MNAzyme (D-S-M) fluorescent biosensor for olfactory marker protein (OMP) detection. A. Workflow for detection of OMP in nasal swab. B. Isothermal Self-Primer EXPonential Amplification Reaction (SPEXPAR) amplification. C. The production of fluorescent signal by Multicomponent Nuclear Acid Enzyme (MNAzyme). The signal of OMP was amplified and changed into the detectable fluorescence signal based on the reactions of SPEXPAR and MNAzyme in the D-S-M fluorescence biosensor. The qualitative or quantitative analysis of OMP can be measured by the analysis of the fluorescence intensity.Image 1.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471671PMC
http://dx.doi.org/10.1016/j.mtbio.2024.101272DOI Listing

Publication Analysis

Top Keywords

dual targets
8
targets driven
8
fluorescent biosensor
8
spexpar mnazyme
8
olfactory marker
8
marker protein
8
mnazyme d-s-m
8
magnetic nanobead
4
nanobead assisted
4
assisted dual
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!