Caproic acid production from short-chained carboxylic acids produced by arrested anaerobic digestion of green waste: Development and optimization of a mixed caproic acid producing culture.

Bioresour Technol

Bioproducts, Science, and Engineering Laboratory, Washington State University, Tri-Cities, 2710, Crimson Way, Richland, WA 99354, USA; Biological System Engineering Department, Washington State University, L. J. Smith Hall, Pullman, WA 99164, USA; Voiland School of Chemical Engineering and Bioengineering, Washington State University, Wegner Hall, Pullman, WA 99164, USA. Electronic address:

Published: December 2024

Caproic acid is an important compound for producing a variety of chemicals and a potential precursor for Sustainable Aviation Fuels (SAF). This study aimed at developing a stable mixed culture producing caproic acid using short-chain carboxylic acids (SCCAs) derived from arrested anaerobic digestion (AAD) of green waste and optimizing chain elongation (CE) conditions. The results showed that the mixed culture was dominated by Clostridium sensu stricto 12 (53.09 %), optimally producing caproic acid at 37 °C, pH 7.20, with a 15 % inoculum concentration. Under these conditions, 75.59 % of the consumed electrons contributed to caproic acid formation. Gradual ethanol feeding in bioreactor experiments resulted in caproic acid titer of 13.36 g/L, with carbon conversion efficiency (CCE) of 81.91 % and electron transfer efficiency (ETE) of 76.39 %, surpassing batch experiments without gradual ethanol addition. These findings demonstrate that the effectiveness of a mixed culture can be improved, resulting in increased caproic acid production.

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http://dx.doi.org/10.1016/j.biortech.2024.131573DOI Listing

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