AI Article Synopsis
- Metronidazole (MNZ) and ibuprofen (IBF) are often illegally added to functional soft drinks, posing health risks to consumers, highlighting the need for effective detection methods.
- This study developed DNA aptamers for MNZ (MNZ-1) and IBF (IBF-1), with specific binding strengths, allowing for selective detection in mixtures of drugs.
- A label-free fluorescent detection method using ThT was created, achieving low detection limits for both additives, paving the way for potential use in food and environmental monitoring.
Article Abstract
To enhance the effects of some functional soft drinks, drugs, especially metronidazole (MNZ) and ibuprofen (IBF), are often illegally added. This poses a serious threat to the health of consumers. Therefore, developing simple and rapid detection methods for these additives is crucial. In this study, DNA aptamers of metronidazole and ibuprofen were selected using the library-immobilized method. The best aptamer for metronidazole, named MNZ-1, has a dissociation constant () value of 4.9 μM and the aptamer for ibuprofen, named IBF-1, shows a of 9.3 μM, as determined by the thioflavin T (ThT) fluorescence assay. The values measured using isothermal titration calorimetry (ITC) were 17.0 μM and 66.7 μM for these two aptamers, respectively. Selectivity experiments indicate that MNZ-1 demonstrates very weak binding to structurally similar drugs, whereas IBF-1 exhibits binding capability to some structurally similar compounds comparable to ibuprofen, enabling the simultaneous detection of these types of drugs. Neither MNZ-1 nor IBF-1 binds to other common drugs. Using ThT, a label-free fluorescent detection method was developed for metronidazole and ibuprofen in soft drinks, showing limits of detection (LODs) of 0.6 μM and 4.7 μM, respectively. Owing to their small size and well-defined secondary structures, these aptamers are expected to be utilized in analytical applications for food and environmental monitoring.
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Article Synopsis
- Metronidazole (MNZ) and ibuprofen (IBF) are often illegally added to functional soft drinks, posing health risks to consumers, highlighting the need for effective detection methods.
- This study developed DNA aptamers for MNZ (MNZ-1) and IBF (IBF-1), with specific binding strengths, allowing for selective detection in mixtures of drugs.
- A label-free fluorescent detection method using ThT was created, achieving low detection limits for both additives, paving the way for potential use in food and environmental monitoring.
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