AI Article Synopsis

  • Lung cancer has a poor prognosis, but recent studies suggest that inducing ferroptosis, a form of cell death, could be a promising treatment approach.
  • This study specifically investigates oleic acid (OA), the main component of Brucea javanica oil (BJO), and its effects on lung cancer cell lines A549 and H1299.
  • Findings indicate that OA inhibits cancer cell growth and migration, suppresses SDC4 expression, and promotes ferroptosis by altering the levels of several key proteins involved in this process, highlighting its potential for lung cancer therapy.

Article Abstract

Introduction: As a common malignancy, lung cancer has a relatively poor prognosis and a low survival rate. In recent years, ferroptosis, as an emerging filed, has great promise in the potential treatment of cancer. Brucea javanica oil (BJO) is often used to treat various cancers. Oleic acid (OA) is the main ingredient of BJO. In this study, we investigated the role and molecular mechanism of OA in lung cancer treatment by promoting ferroptosis.

Methods: In this study, A549 cells and H1299 cells were used for in vitro experiments, and a CCK-8 test, scratch test, and MTT experiment were carried out. We examined reactive oxygen species (ROS), the JC-1 probe, glutathione (GSH) expression, lipid peroxidation, SDC4 mRNA levels, and ACSL4, SLC7A11, GPX4, and SDC4 protein levels.

Results: The results showed that OA could inhibit the proliferation and migration of A549 cells and H1299 cells, SDC4 was a potential therapeutic target of OA against lung cancer, and OA treatment significantly inhibited the expression of SDC4 in A549 cells and H1299 cells. OA induces ferroptosis in A549 cells and H1299 cells, decreases GSH levels, increases lipid peroxidation levels, and decreases SDC4 mRNA expression; in addition, OA upregulates ACSL4 expression and decreases SLC7A11, GPX4, and SDC4 expression.

Conclusion: This study confirmed that OA could inhibit SDC4 expression and promote the occurrence of ferroptosis in A549 cells and H1299 cells through the GPX4/ACSL4 pathway, providing an effective basis for the use of drugs targeting ferroptosis in lung cancer treatment.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471947PMC
http://dx.doi.org/10.1111/crj.70014DOI Listing

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