AI Article Synopsis
- The study focuses on the role of mobile genetic elements in spreading biocide and antibiotic resistance among bacteria, specifically the qacEΔ1 gene that provides resistance to quaternary ammonium compounds (QACs).
- Researchers developed a TaqMan real-time PCR assay to detect the qacEΔ1 gene in Gram-negative bacteria, which can accurately identify even low quantities (as few as 80 copies) without false results.
- This assay serves as a rapid, sensitive, and specific method for monitoring QAC resistance, tracking the spread of class 1 integrons, and predicting multidrug-resistant (MDR) traits in Gram-negative bacteria.
Article Abstract
The transfer of biocide and antibiotic resistance genes by mobile genetic elements is the most common mechanism for rapidly acquiring and spreading resistance among bacteria. The qacEΔ1 gene confers the resistance to quaternary ammonium compounds (QACs). It has also been considered a genetic marker for the presence of class 1 integrons associated with multidrug-resistant (MDR) phenotypes in Gram-negative bacteria. In this study, a TaqMan real-time PCR assay was developed to detect the qacEΔ1 gene in Gram-negative bacteria. The assay has a detection limit of 80 copies of the qacEΔ1 gene per reaction. No false-positive or false-negative results have been observed. Simultaneous amplification and detection of the 16S rRNA gene is performed as an endogenous internal amplification control (IAC). The TaqMan real-time PCR assay developed is a rapid, sensitive, and specific method that could be used to monitor resistance to QACs, the spread of class 1 integrons, and the prediction of associated MDR phenotypes in Gram-negative bacteria.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2024.107054 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bioinformatics Based Drug Repurposing Approach for Breast and Gynecological Cancers: Genes Address Common Hub Genes and Drugs.
Eur J Breast Health
January 2025
Department of Biomedical Engineering, Faculty of Engineering, İzmir University of Economics, İzmir, Turkey.
Objective: The prevalence of breast cancer and gynaecological cancers is high, and these cancer types can occur consecutively as secondary cancers. The aim of our study is to determine the genes commonly expressed in these cancers and to identify the common hub genes and drug components.
Materials And Methods: Gene intensity values of breast cancer, gynaecological cancers such as cervical, ovarian and endometrial cancers were used from the Gene Expression Omnibus database Affymetrix Human Genome U133 Plus 2.
Mitochondrial control region sequences show high genetic connectivity in the brownstripe snapper, (Quoy and Gaimard, 1824) from the east coast of Peninsular Malaysia.
J Genet
January 2024
1Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
The Brownstripe Snapper, (Quoy and Gaimard, 1824) is a commercially important snapper extensively caught in Malaysia. We examined genetic diversity, population connectivity, and historical demographics of the , off the eastern coast of peninsular Malaysia based on an 817 bp region of the mtDNA control region sequences. Maximum likelihood gene trees demonstrated that the populations under study had limited structuring and formed a single panmictic population that lacks support for internal clades.
View Article and Find Full Text PDFThe complete mitochondrial genome of the deep-sea methanotrophic sponges and : leveraging 'waste' in metagenomic data.
J Genet
January 2024
Programa de Pos-graduacao em Ciencias Biologicas (Genetica), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
A significant proportion of next-generation sequencing (NGS) data ends up not being used since they comprise information out-of-scope of the primary studies. This 'waste' of potential can be harnessed to explore organellar genomes, such as the mitochondrial DNA, and be used for evolutionary, conservation and biodiversity research. We present the complete mitochondrial genomes of the deep-sea methanotrophic sponges and (Demospongiae, Poecilosclerida) retrieved from previously published whole metagenome sequencing data.
View Article and Find Full Text PDFTransitioning From Nusinersen to Risdiplam for Spinal Muscular Atrophy in Clinical Practice: A Single-Center Experience.
Muscle Nerve
January 2025
Center for Gene Therapy, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, USA.
Background: Nusinersen and risdiplam are U.S. Food and Drug Administration (FDA)-approved treatments for spinal muscular atrophy (SMA).
View Article and Find Full Text PDFElongator subunit Elp3 regulates reproduction in Tribolium castaneum by interacting with FOXO.
Insect Sci
January 2025
College of Plant Protection, Yangzhou University, Yangzhou, China.
As the catalytic subunit of the Elongator complex, Elongator protein 3 (Elp3) plays a crucial role in multiple physiological processes, including growth, development and immune responses. Previous studies on Elp3 have focused on Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens (human) or Mus musculus (mouse), whereas there are few reports on Elp3 in agricultural pests. Here, the role of TcElp3 in reproduction in the red flour beetle, Tribolium castaneum, was investigated, and the underlying mechanisms were explored.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!