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Structural characteristics of a purified Evodiae fructus polysaccharide and its gastroprotection and relevant mechanism against alcohol-induced gastric lesions in rats. | LitMetric

AI Article Synopsis

  • The study focuses on Evodiae fructus polysaccharide (EFP) and its purified fraction EFP-2-1, which has gastroprotective effects against alcohol-induced stomach lesions but needed further characterization.
  • EFP-2-1 was analyzed using advanced techniques and found to have a specific molecular structure, primarily consisting of rhamnose and other sugars, with a molecular weight of 7.3 kDa.
  • The fraction not only reduced gastric damage but also influenced inflammatory markers, activated specific metabolic pathways, and improved gut microbiota composition, suggesting its potential as a protective agent against alcohol-related gastric issues.

Article Abstract

Evodiae fructus polysaccharide (EFP) has been previously shown to protect against alcohol-induced gastric lesions. However, which and how active fractions in EFP exert gastroprotection remains unclear. This study aimed to characterize the structure of the purified fraction (EFP-2-1) of EFP, and investigate its gastroprotection and underlying mechanisms. EFP-2-1 was obtained through column chromatography, and was characterized using instrumental analytical techniques. Gastroprotective effect of EFP-2-1 was evaluated using alcohol-induced gastric lesions in rats, and its mechanism was explored through proteomics, metabolomics and diversity sequencing. Results showed that EFP-2-1 had a molecular weight of 7.3 kDa, and consisted mainly of rhamnose, galacturonic acid, galactose and arabinose. Its backbone contained HG and RG-I domains, and branched with →5)-α-l-Araf-(1→, α-l-Araf-(1→ and →4)-β-d-Galp-(1→ residues. EFP-2-1 reduced gastric lesions and the levels of MDA, TNF-α and IL-6, activated PPARγ, primarily altered protein digestion and absorption and bile secretion metabolic pathways, regulated gut microbiota like Faecalibaculum and Lachnoclostridium, and increased short-chain fatty acids production. Correlations were observed among the gut microbiota, metabolites and biochemical indexes influenced by EFP-2-1. These findings suggest that EFP-2-1 is an active fraction of EFP for protecting against alcohol-induced gastric lesions, which may be linked to PPARγ activation, gut microbiota and serum metabolism.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2024.136410DOI Listing

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