AI Article Synopsis
- Learning and memory involve long-term potentiation (LTP) of synaptic strength, which requires CaMKII primarily for its structural functions.
- CaMKII binds to the NMDA receptor subunit GluN2B to generate Ca-independent activity that is crucial for an intermediary phase of LTP after initial induction but not for long-term maintenance.
- This study reveals that while the enzymatic activity of CaMKII isn't needed for the quick start of LTP, it plays a role later on, indicating a clear timeline for different phases of LTP expression.
Article Abstract
Learning and memory are thought to require the induction and maintenance of long-term potentiation (LTP) of synaptic strength. LTP induction requires the Ca/calmodulin-dependent protein kinase II (CaMKII) but for structural rather than enzymatic functions. We show that the relevant structural function is regulated by CaMKII binding to the NMDA-type glutamate receptor subunit GluN2B. This binding directly generates Ca-independent autonomous CaMKII activity, and we show that this enzymatic activity is dispensable for LTP induction (within 5 min) but required for a subsequent LTP phase (within 15 min). This requirement for CaMKII activity provides an objective temporal definition for the intermediary phase of LTP expression. Later LTP maintenance may still require structural functions of GluN2B-bound CaMKII but not the resulting enzymatic CaMKII activity or their co-condensation. Thus, autonomous CaMKII activity mediates post-induction LTP but (1) via GluN2B binding, not T286 autophosphorylation, and (2) during the intermediary expression phase rather than for long-term maintenance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563194 | PMC |
| http://dx.doi.org/10.1016/j.celrep.2024.114866 | DOI Listing |
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