AI Article Synopsis

  • The mammary gland is crucial for the development and survival of mammals, with myoepithelial cells playing a key role in milk ejection through contractions triggered by oxytocin.
  • The study utilizes mice with a genetically encoded calcium indicator (GCaMP6f) to visualize changes in intracellular calcium in mammary epithelial cells.
  • The chapter includes a detailed procedure for preparing mammary tissue and primary cells for observation, both in two-dimensional (2D) and three-dimensional (3D) settings.

Article Abstract

The mammary gland has a central role in optimal mammalian development and survival. Contractions of smooth muscle-like basal (or myoepithelial) cells in the functionally mature mammary gland in response to oxytocin are essential for milk ejection and are tightly regulated by intracellular calcium (Ca). Using mice expressing a genetically encoded Ca indicator (GCaMP6f), we present in this chapter a method to visualize at high spatiotemporal resolution changes in intracellular Ca in mammary epithelial cells, both in vitro (2D) and ex vivo (3D). The procedure to optimally prepare mammary tissue and primary cells is presented in detail.

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http://dx.doi.org/10.1007/978-1-0716-4164-4_15DOI Listing

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