AI Article Synopsis
- Intracellular calcium plays a crucial role in regulating solute transport in kidney cells, and issues with calcium signaling can contribute to kidney disease.
- Using advanced imaging techniques on transgenic mice with fluorescent markers, researchers can examine calcium signals in specific parts of the renal tubule and their interaction with other cellular processes.
- The chapter includes a detailed protocol for conducting this innovative research method, allowing real-time observation of calcium activity during physiological changes or disease conditions.
Article Abstract
Intracellular calcium is an important regulator of solute transport in renal epithelial cells, and disordered calcium signaling may underlie the pathogenesis of certain kidney diseases. Intravital multiphoton imaging of the kidney in transgenic mice expressing highly sensitive fluorescent reporters allows detailed study of calcium signals within different specialized segments of the renal tubule and how these are integrated with other cellular processes. Moreover, changes in activity can be observed in real time in response to physiological interventions or disease-causing insults. In this chapter, we will provide a detailed protocol for performing this powerful research technique.
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| http://dx.doi.org/10.1007/978-1-0716-4164-4_14 | DOI Listing |
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