AI Article Synopsis
- Ascochyta blight is a significant issue for chickpea production worldwide, and controlling it typically involves fungicide use, which can impact crop quality.
- Traditional diagnostic methods for detecting the Ascochyta rabiei pathogen require specialized equipment and trained personnel, making them time-consuming and complex.
- A new rapid detection method using a molecular beacon probe has been developed, allowing for accurate pathogen detection in just 30 minutes and holding promise for future in-field applications despite being initially lab-based.
Article Abstract
Ascochyta blight is a major biotic stress that limits chickpea production globally. Fungicide application remains one of the effective control measures for the endemic spread. Due to the serious threat that synthetic fungicides pose to crop quality, early diagnosis of the pathogen is imperative. Whilst there have previously been several conventional lab-based diagnostic methods developed for early detection of Ascochyta rabiei, they require long assay times, specialised equipment and facilities, and trained personnel to process the samples. To overcome this challenge, a rapid amplification-free detection assay using a molecular beacon probe was developed. The method consists of a simple assembly assay that accurately detects pathogen within 30 min. The developed assay is species-specific and has a similar sensitivity level as conventional amplification-based methods. Although it is still a lab-based technique, considering the simplicity of the assay, it has a great potential to be developed further as a reliable in-field diagnostic device for early detection and quantification of fungal pathogen spores.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470000 | PMC |
| http://dx.doi.org/10.1038/s41598-024-74564-5 | DOI Listing |
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