AI Article Synopsis
- A novel polysaccharide called SSP-3a was extracted from the stem and leaves of Scutellaria baicalensis, characterized by a high molecular weight of 1.83 × 10 Da and composed mainly of mannose, glucuronic acid, glucose, and xylose.
- Structural analysis confirmed SSP-3a's acidic nature and its β-configuration pyranose structure.
- Immunomodulatory tests indicated that SSP-3a enhances RAW264.7 cell proliferation and phagocytosis and activates MAPKs and NF-κB signaling pathways, suggesting its potential use in food and pharmaceutical applications.
Article Abstract
In our research, a novel polysaccharide (named SSP-3a) with uniform molecular weight was extracted from Scutellaria baicalensis stem-leaf. The structural analysis revealed that SSP-3a was an acidic polysaccharide with a heavy average molecular weight of 1.83 × 10 Da. By HPLC, the primary constituents of SSP-3a were mannose (11.60 %), glucuronic acid (42.99 %), glucose (23.43 %), and xylose (22.04 %). According to FT-IR and H NMR analysis, it was confirmed to be a β-configuration pyranose with a CO stretching vibrational peak. The immunomodulation results also showed that SSP-3a not only significantly promoted RAW264.7 cell proliferation and phagocytosis, but also stimulated the release of NO and cytokines. Furthermore, mechanistic studies suggested that SSP-3a had the ability to trigger MAPKs and NF-κB immunological signaling pathways via TLR4 receptors. The findings suggested that SSP-3a might be a beneficial active component for the food and pharmaceutical industries.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.136409 | DOI Listing |
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