PI4KB is an essential host factor for duck hepatitis a virus 1 replication and translation.

Duck hepatitis A virus 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. Phosphatidylinositol 4-kinases (PI4Ks) are important for viral replication, and different viruses have different strategies to hijack PI4Ks. To date, few studies have investigated the DHAV-1 life cycle; thus, whether PI4Ks are required for DHAV-1 replication has not been reported. In this study, we found that the PI4KB protein, a PI4K, promoted the replication and translation of DHAV-1, and the 2A2, 2C, 2BC, 3A, 3AB, 3D, and 3CD proteins of DHAV-1 were able to interact with the PI4KB protein. Amino acids 101-120 of the 2A2 protein is the region where the 2A2 protein interacts with the PI4KB protein.