AI Article Synopsis

  • Infectious laryngotracheitis (ILT) is a major issue for the poultry industry, caused by the infectious laryngotracheitis virus (ILTV), leading to animal welfare concerns and economic losses.
  • This study compared the interactions between a glycoprotein G deletion mutant vaccine strain of ILTV and its wild-type strain in chicken cell cultures, revealing distinct gene expression patterns in different cell types.
  • Results indicated that the type of chicken cells used had a bigger impact on host and viral gene transcription than the presence or absence of the gG gene, emphasizing the need for careful cell-line choice in future research on these virus interactions.

Article Abstract

Infectious laryngotracheitis (ILT) remains a significant concern for the poultry industry worldwide due to its impact on animal welfare and its substantial economic consequences. The disease is caused by the alphaherpesvirus, infectious laryngotracheitis virus (ILTV). This study investigated in vitro host-virus interactions of a glycoprotein G (gG) deletion mutant vaccine strain of ILTV (ΔgG ILTV), and its parent wild-type strain (CSW-1 ILTV). Inoculations were performed separately for the two strains of ILTV using both a primary (chicken embryonic kidney, CEK) and a continuous culture (leghorn male hepatoma, LMH) of chicken cells. Transcriptome analysis was performed at 12 hours post infection. Each cell-type displayed distinct effects on host and viral gene transcription, with a greater number of viral and host genes differentially transcribed in CEK cells and LMH cells, respectively. Both cell-types infected with either strain demonstrated enrichment of pathways related to signalling, and gene ontologies (GO) associated with chemotaxis. Infection with either strain upregulated both SOCS proteins and certain proto-oncogenes, which may contribute to prolonged viral persistence by promoting immunosuppression and preventing apoptosis, respectively. Patterns of gene transcription related to cytokines, chemokines, endosomal TLRs, and interferon responses, as well as pathways associated with histone acetylation, transport, and extracellular matrix organization were similar within each cell type, regardless of the viral strain. In CEK cells, GO terms and pathways were downregulated uniquely after CSW-1 ILTV infection, indicating a viral-strain specific effect in this cell-type. Overall, this study highlights that the observed differences in host and ILTV gene transcription in vitro were more strongly influenced by the cell-types used rather than the presence or absence of gG. This underscores the importance of cell-line selection in studying host-virus interactions and interpreting experimental results.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469545PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0311874PLOS

Publication Analysis

Top Keywords

host-virus interactions
12
gene transcription
12
iltv
8
strains iltv
8
iltv primary
8
infectious laryngotracheitis
8
csw-1 iltv
8
cek cells
8
strain
5
transcriptomic analyses
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!