AI Article Synopsis
- Serum response factor (SRF) is crucial for various cellular processes like growth and movement, and it's vital for early embryonic development, specifically for forming certain structures.
- SRF interacts with proteins CTCF and cohesin at specific genomic regions, helping to form long-range chromatin loops and manage the insulation of topologically associating domains (TADs).
- In embryonic stem cells, SRF works with factors like SOX2 and NANOG to create three-dimensional structures that support pluripotency, indicating it has important roles in organizing chromatin at higher levels.
Article Abstract
Serum response factor (SRF) is a transcription factor essential for cell proliferation, differentiation, and migration and is required for primitive streak and mesoderm formation in the embryo. The canonical roles of SRF are mediated by a diverse set of context-dependent cofactors. Here, we show that SRF physically interacts with CTCF and cohesin subunits at topologically associating domain (TAD) boundaries and loop anchors. SRF promotes long-range chromatin loop formation and contributes to TAD insulation. In embryonic stem cells (ESCs), SRF associates with SOX2 and NANOG and contributes to the formation of three-dimensional (3D) pluripotency hubs. Our findings reveal additional roles of SRF in higher-order chromatin organization.
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| http://dx.doi.org/10.1016/j.celrep.2024.114846 | DOI Listing |
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