AI Article Synopsis
- Glioblastoma (GBM) is an aggressive brain tumor with poor survival rates, and RBMS1 is a protein that may influence its progression.
- This study utilized various laboratory techniques to analyze RBMS1 expression and its effects on GBM cell behaviors, like proliferation, apoptosis, and invasion.
- The findings indicated that reducing RBMS1 levels decreased GBM cell malignancy and promoted ferroptosis, a type of cell death, suggesting RBMS1 plays a significant role in GBM progression.
Article Abstract
Background: Glioblastoma (GBM) is a brain tumor characterized by the highest malignancy and the poorest prognoses. RNA binding motif single strand interacting protein 1 (RBMS1) has been implicated to be involved in various cancer progression. This study was conceived to explore the role and the mechanism of RBMS1 in GBM.
Materials: RT-qPCR and western blot were used to evaluate RBMS1 expression and examine the transfection efficiency of sh-RBMS1. Cell proliferation was detected using CCK-8 assay and colony formation assay while cell apoptosis was detected with flow cytometry. Cell migration and invasion were detected with wound healing and transwell assay. The activities of MMP2 and MMP9 were detected using gelatin zymography. Western blot was used to measure proliferation-, apoptosis-, ferroptosis- and EMT-related proteins. Lipid peroxidation was detected with TBARS Assay Kit and lipid ROS was detected with a BODIPY 581/591 C11 kit. The total iron level was detected using corresponding assay kits.
Results: According to GEPIA database, RBMS1 expression was upregulated in GBM and the present study found that RBMS1 expression was upregulated in GBM cells. After interfering RBMS1, GBM cell proliferation, migration, invasion and EMT process were inhibited while cell apoptosis and ferroptosis were promoted. However, ferroptosis inhibitor Fer-1 partially counteracted the protective effects of RBMS1 knockdown on GBM.
Conclusion: Collectively, this study revealed that RBMS1 silence inhibited the malignant progression of GBM possibly through ferroptosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469991 | PMC |
| http://dx.doi.org/10.1007/s12672-024-01430-1 | DOI Listing |
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