Introduction: Melanoma is still one of the deadliest cancers whose prevalence has increased in recent decades. Today, many polysaccharides and their bioactive compounds have been of special importance in modern biotechnology. They have various biological and therapeutic properties. they can regulate and strengthen the immune system, lower blood pressure and cholesterol, and reduce bacterial and viral infections. According to studies, these compounds also have antitumor properties. we investigated the cytotoxic effects of obtained from solid-state fermentation (SSF) of edible medicinal mushroom on cancerous skin cells.
Materials And Methods: The mitochondria were isolated from melanoma cells via differential centrifugation and treated with various concentrations (30, 45, 60, 90, 120, and 240 µg/ml) of extract. Then, they were subjected to MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release, and flow cytometry assays.
Results: The results of the MTT assay showed that IC50 of extract was 60 μg/ml, and it induced a selectively significant (P < 0.05) concentration-dependent decrease in the SDH activity in cancerous skin mitochondria. At higher concentrations, no such effect was observed. The ROS results also showed that 30, 45, and 60 µg/ml concentrations of extract significantly increased ROS. However, no such effect was observed at higher concentrations. MMP decline and the release of cytochrome c in cancer groups mitochondria and swelling were significantly increased at 30, 45, and 60 µg/ml compared to the control group. At higher concentrations, no such effect was observed. extract at 60 µg/ml concentration increased apoptosis on melanoma cells, while it had no effect on control non-tumour cells.
Discussion And Conclusion: Based on these results, extract at 30, 45, and 60 µg/ml showed a cytotoxic effect, while no such effect was observed at higher concentrations. Overall, it seems that has antioxidant and free radical scavenging effects on cancer cells at higher concentrations.
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http://dx.doi.org/10.1080/15569527.2024.2410355 | DOI Listing |
Ann Surg Oncol
December 2024
Division of Breast Surgery, Department of Surgery, Linkou Chang Gung Memorial Hospital, Taoyüan, Taiwan, R.O.C..
Background: We investigated the perioperative outcome and oncologic safety of performing nipple-sparing mastectomy (NSM) through a single axillary incision (NSM-SAI) compared with performing NSM through a conventional incision (NSM-C).
Methods: We retrospectively reviewed 725 patients who underwent NSM for breast cancer between January 2010 and December 2023; 333 patients who underwent NSM with immediate reconstruction (IR) were enrolled. Surgical outcomes and oncologic outcomes of NSM-C (n = 184), NSM performed through SAI with a freehand approach (NSM-SAI-F; n = 92), and with an endoscopic approach (NSM-SAI-E; n = 57) were demonstrated.
J Cutan Med Surg
December 2024
The Ottawa Hospital Research Institute, The Ottawa Hospital, Ottawa, ON, Canada.
J Am Acad Dermatol
December 2024
Weill Cornell Medicine, Department of Dermatology, New York, NY. Electronic address:
Lancet Microbe
December 2024
Amsterdam University Medical Centres, Amsterdam, Netherlands; Department of Global Health and Amsterdam Institute for Global Health and Development, Amsterdam, Netherlands.
Background: Tuberculosis vaccine trials using disease as the primary endpoint are large, time consuming, and expensive. An earlier immunological measure of the protection against disease would accelerate tuberculosis vaccine development. We aimed to assess whether the effectiveness of the Bacillus Calmette-Guérin (BCG) vaccine for prevention of Mycobacterium tuberculosis infection was consistent with that for prevention of tuberculosis disease.
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