AI Article Synopsis

  • This study examines the osteogenic effects of soybean peptide VVELLKAFEEKF (SOP) and its relationship with TGF-β1 in promoting bone cell activity.
  • SOP encourages proliferation, differentiation, and mineralization of osteoblasts in a dose-dependent manner through the activation of specific signaling pathways (p38-MAPK and Smad2/3).
  • Additionally, SOP has shown potential in improving bone density and function in glucocorticoid-induced osteoporosis models in zebrafish, suggesting its possible use as a functional food to combat osteoporosis.

Article Abstract

The aim of this study was to determine the osteogenic activity and mechanism of soybean peptide VVELLKAFEEKF (SOP) and the potential relationship between SOP and transforming growth factor-β1 (TGF-β1). The results show that SOP promotes MC3T3-E1 cell proliferation by altering cell progression. SOP induced cell differentiation and mineralization in a dose-dependent manner at 0.7-7 μM. Moreover, SOP stimulates osteoblast differentiation, which may be achieved through the activation of p38-MAPK and Smad2/3 signaling pathways. Furthermore, treatment with a TβRI inhibitor (SB525334) inhibited the phosphorylation levels of p38 and Smad2/3, which indicates the involvement of TβRI in the process of osteoblast differentiation caused by SOP. Besides, in non-FBS-cultured MC3T3-E1 cells, SOP and TGF-β1 promoted the phosphorylation of Smad2/3 and alkaline phosphatase (ALP) activity, but the effect was lost when SOP was incubated separately, indicating that SOP stimulated osteoblast differentiation by promoting TGF-β1 activity. , SOP significantly restores bone mineral density loss and behavioral deficits in a model of glucocorticoid-induced osteoporosis (GIOP) in zebrafish. These results suggest that SOP may have the function of promoting bone remodeling and may be used as a potential active factor for functional food development to prevent osteoporosis.

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Source
http://dx.doi.org/10.1021/acs.jafc.4c04882DOI Listing

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