AI Article Synopsis
- Intravital deep bone marrow imaging helps study cell behavior but is difficult and needs less invasive techniques; this research uses a high-energy 1650 nm laser for three-photon microscopy in mouse bones.* -
- The study observes cell movement patterns, finding that plasma cells exhibit two distinct migratory behaviors and uses third harmonic generation (THG) as a label-free marker for cellular structures linked to protein synthesis.* -
- Results indicate that about one-third of plasma cells have strong THG signals suggesting active antibody secretion, and there's an inverse relationship between cell movement and THG signals, providing insight into how the bone marrow environment affects cell function.*
Article Abstract
Intravital deep bone marrow imaging is crucial to studying cellular dynamics and functions but remains challenging, and minimally invasive methods are needed. We employed a high pulse-energy 1650 nm laser to perform three-photon microscopy , reaching ≈400 μm depth in intact mouse tibia. Repetition rates of 3 and 4 MHz allowed us to analyze motility patterns of fast and rare cells within unperturbed marrow and to identify a bi-modal migratory behavior for plasma cells. Third harmonic generation (THG) was identified as a label-free marker for cellular organelles, particularly endoplasmic reticulum, indicating protein synthesis capacity. We found a strong THG signal, suggesting high antibody secretion, in one-third of plasma cells while the rest showed low signals. We discovered an inverse relationship between migratory behavior and THG signal, linking motility to functional plasma cell states. This method may enhance our understanding of marrow microenvironment effects on cellular functions.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466647 | PMC |
| http://dx.doi.org/10.1016/j.isci.2024.110985 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!