Biochemical, toxicological, and microbiological assessment of calcined poultry manure for potential use as bone scaffold material.

The biosafety of thermally calcined poultry manure as a hydroxyapatite source for potential use as bone-making material was investigated in this study. In vitro assays were used to determine the sensitivity of the antioxidant properties to the thermal calcination temperature used to process the poultry manure (750, 800, and 850 ). The effect of the extract of both calcined poultry manure (local) and analytical grade hydroxyapatite (foreign) at various concentrations of 100%-25 % inclusion at (100 mg/kg) body weight intubation for 21 days on kidney, liver, and serum of animal model used was assessed. The results show that the thermally calcined poultry manure-derived hydroxyapatite generally possessed good antioxidant properties with the poultry manure treated at 750 having the most promising antioxidant properties compared to those treated at 800 and 850 , and hence a more likely improved anti-toxicity potential. The various blends of the analytical high-grade hydroxyapatite and thermally calcined poultry manure hydroxyapatite samples are safe compared to the normal control rats with regards hepatic function and renal function parameters with the equal blend of analytical high grade and thermally calcined poultry manure-derived hydroxyapatite (1:1) possessing the lowest activity concentrations. In addition, the enzymatic (glutathione peroxidase) and non-enzymatic (reduced glutathione) antioxidant concentrations of the experimental animals administered the varied compositions of the analytical high grade and thermally calcined poultry manure-derived hydroxyapatite, were lower when compared to normal control rats. The microbiological evaluation suggests that the calcined poultry manure inclusion at various concentrations could not pose a negative effect on various pathology in the liver, kidney, and blood.