AI Article Synopsis
- The research investigates how scopolamine (SCO) could help treat osteoporosis and the mechanisms behind it, using a rat model with ovariectomy (OVX) to simulate the condition.
- Four groups of rats were used in the study: a control group, an OVX group, and two groups receiving SCO, with one also treated with an Nrf2 inhibitor (ML385).
- Results showed that SCO improved bone health by reducing oxidative stress and enhancing certain protein expressions linked to bone formation, while ML385 blocked these positive effects, indicating the involvement of the Nrf2 signaling pathway in SCO's therapeutic action.
Article Abstract
The aim of our research is to investigate the therapeutic effects of scopolamine (SCO) on osteoporosis and to explore the underlying mechanism. To study osteoporosis, we established an ovariectomy (OVX) model. The rats were divided into four groups: sham operation, OVX, OVX+SCO, and OVX+SCO+ML385 (Nrf2 inhibitor). ELISA, Realtime PCR, Western blot, and kits were utilised to assess the expression of related proteins and substances. The OVX rats exhibited significant weight gain, reduced bone volume, destruction of trabecular and cortical bone microstructure, decreased expression of ALP, OCN, OPN, COL1A1, Runx2, Nrf2 proteins, and CAT, SOD, GST, GPX levels while increased expression of TRAP protein and ROS levels. SCO was able to restore these indices in OVX rats, while ML385 blocked the effects of SCO. In conclusion, SCO inhibits oxidative stress response to exert therapeutic effects on osteoporosis by activating the Nrf2 signalling pathway.
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| http://dx.doi.org/10.1080/14786419.2024.2411725 | DOI Listing |
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