AI Article Synopsis
- B cells are crucial for the immune response as they produce antibodies, and there's ongoing research into their development, but results vary due to different species and methods used in studies.
- The current research focuses on characterizing B cells from human sources, identifying that around 50% of IgG-producing B cells in blood and up to 90% in tonsils are "atypical" memory B cells.
- The study also explored how cytokines IL-21 and TGF-β1 affect the production and expression of antibodies on B cells, revealing significant differences in their effects.
Article Abstract
B cells play a key role in humoral immune responses by producing antibodies. Although there are numerous research on memory B cells definition markers and cytokines on B cell development, different studies have yielded contradictory conclusions due to species studied, the different cells and stimulating agents used. In the current study, we conducted a detailed characterization of B cells in human CBMCs, PBMCs and tonsil, including expression of Igs, activation and memory markers. Furthermore, we found that considerable amounts of IgA and IgG were expressed by CD27 B cells. These "Atypical" memory B cells corresponded to approximately 50% of IgG and IgAB cells in blood, this proportion even reached 90% in tonsil. In addition, we investigated the effect of IL-21 and TGF-β1 on the membrane-bound form and secreted form of Igs using PBMCs and purified blood B cells. There were actual differences between the effect of cytokines on Igs secretion and surface expression. Our study will be helpful to advance the knowledge and understanding of humoral memory.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11467254 | PMC |
| http://dx.doi.org/10.1038/s41598-024-75636-2 | DOI Listing |
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