AI Article Synopsis
- The connection between APOBEC3 cytosine deaminases and cancer mutations has been highlighted, revealing a significant gap in available drugs to inhibit these enzymes.
- A new Real-time APOBEC3-mediated DNA Deamination (RADD) assay has been developed, offering a quick and effective way to measure DNA deamination through fluorescence.
- This chapter outlines how to use the RADD assay for studying APOBEC3 enzymes and scouting for potential inhibitors, enhancing existing research tools in the field.
Article Abstract
In recent years, the connection between APOBEC3 cytosine deaminases and cancer mutagenesis has become ever more apparent. This growing awareness and lack of inhibitory drugs has created a distinct need for biochemical tools that can be used to identify and characterize potential inhibitors of this family of enzymes. In response to this challenge, we have developed a Real-time APOBEC3-mediated DNA Deamination (RADD) assay. The RADD assay provides a rapid, real-time fluorescence readout of APOBEC3 DNA deamination and serves as a crucial addition to the existing APOBEC3 biochemical and cellular toolkit. This method improves upon contemporary DNA deamination assays by offering a more rapid and quantifiable readout as well as providing a platform that is readily adaptable to a high-throughput format for inhibitor discovery. In this chapter we provide a detailed guide for the usage of the RADD assay for the characterization of APOBEC3 enzymes and potential inhibitors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11483159 | PMC |
| http://dx.doi.org/10.1016/bs.mie.2024.08.001 | DOI Listing |
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