AI Article Synopsis
- * U937 cells were modified using RNA interference and overexpression techniques to examine the effects of Tollip on cell behavior and TB infection in a lab setting.
- * Results indicate that Tollip can negatively regulate factors in the TLR4 pathway, suggesting its potential as a biomarker for early TB diagnosis.
Article Abstract
The emergence of drug-resistant mycobacterium tuberculosis (MTB, or TB) strains has led to an increasing incidence of TB. Spinal tuberculosis is the most common extrapulmonary tuberculosis. In the present study, tollip, a negative feedback regulatory factor in TLR4 signaling pathway was chosen based on previous studies on osteoarticular tuberculosis. U937 cells were transfected with recombinant lentivirus containing shRNA (RNA interference, RNAi) or overexpression vector containing Tollip gene and tested in vitro. The expression levels of Tollip and TLR4 were detected by Real-time PCR and immunofluorescence techniques, and the cell morphology and infection effect were observed by DAPI staining. The results suggested that Tollip gene could negatively inhibit the expression of related factors in TLR4 signaling pathway, and thus is a potential biomarker for early diagnosis.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.136458 | DOI Listing |
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