The extremolytes ectoine and hydroxyectoine are osmolytes found in extremophilic microorganisms. They are stabilisers of proteins and other macromolecules, including DNA and lipids. The aim of the study was to investigate the effect of the additives on the heat-induced aggregation of mink growth hormone as a model protein. The first-order rate constants of protein aggregation were determined at 60 °C depending on the additive concentration and pH of the solution. The onset temperature of aggregation was also recorded using a circular dichroism spectropolarimeter. The study showed that the effect of the additives depended on the pH of the solution. The first-order rate constants of aggregation were lower when the protein molecule had a negative charge. The effect also depended on the structure of the extremolyte itself. When the protein molecule was positively charged, hydroxyectoine destabilised the mink growth hormone molecule and promoted the aggregation. The different effects of the additives were determined by the different interactions with the protein molecules, as shown by circular dichroism measurements and previously by fluorescence spectroscopy. Therefore, when using ectoine or hydroxyectoine for protein formulation, the effect of the additive should be carefully analysed for each protein individually.
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http://dx.doi.org/10.1016/j.biochi.2024.10.006 | DOI Listing |
Microbiol Resour Announc
December 2024
Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.
Vibrio diabolicus 3098 is a marine halophile, a member of the . The draft whole-genome sequence is 5.17 Mb and has 4,829 predicted coding sequences divided into two chromosomes and a plasmid.
View Article and Find Full Text PDFJ Environ Manage
December 2024
Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina s/n., 47011, Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n., 47011, Valladolid, Spain. Electronic address:
Today, the use of biogas to produce more sustainable bioproducts is attracting an increasing attention in the quest for a circular economy. This work aims at optimizing the biosynthesis of high value bioproducts such as ectoine and hydroxyectoine from methane using a high mass transfer Taylor flow reactor and a methanotrophic consortium. The influence of the gas residence time (30-240 min) and concentration of microorganisms (0.
View Article and Find Full Text PDFBiochimie
October 2024
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania. Electronic address:
The extremolytes ectoine and hydroxyectoine are osmolytes found in extremophilic microorganisms. They are stabilisers of proteins and other macromolecules, including DNA and lipids. The aim of the study was to investigate the effect of the additives on the heat-induced aggregation of mink growth hormone as a model protein.
View Article and Find Full Text PDFBMC Chem
October 2024
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia.
Bioresour Technol
December 2024
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China. Electronic address:
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