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Construction of a full-length infectious cDNA clone of zucchini tigre mosaic virus infecting snake gourd and genetic diversity analysis based on complete genome sequences of ZTMV isolates. | LitMetric

AI Article Synopsis

  • - Zucchini tigre mosaic virus (ZTMV) is a positive-sense RNA virus from the Potyvirus genus, which infects various plants like snake gourd and zucchini.
  • - Researchers created a full-length infectious cDNA clone of ZTMV to study its infection capabilities and sequenced its genome, finding a close genetic relation to the ZTMV-TW strain.
  • - Analysis of 12 ZTMV isolates revealed high variability in the P1 gene, with overall negative selection on all genes but some positive selection at certain sites within the P1 gene itself.

Article Abstract

Zucchini tigre mosaic virus (ZTMV) is a positive-sense single-stranded RNA virus belonging to the genus Potyvirus. In this study, a full-length infectious cDNA clone of a ZTMV strain infecting snake gourd (Trichosanthes cucumerina var. anguina L.) was constructed and shown to infect snake gourd, chieh-qua, zucchini, ridge gourd, and bitter melon. The complete genome sequence of ZTMV-FS7 (PP291701) showed the highest nucleotide sequence similarity to ZTMV-TW (86.2% identity). Genetic diversity analysis of 12 ZTMV isolates showed that the P1 gene had the highest variability. Selection pressure analysis indicated that all of the ZTMV genes were under negative selection. However, some sites, particularly within the P1 gene, were under positive selection.

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Source
http://dx.doi.org/10.1007/s00705-024-06152-xDOI Listing

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