Energy-dispersive Laue diffraction (EDLD) is a powerful method to obtain position-resolved texture information in inhomogeneous biological samples without the need for sample rotation. This study employs EDLD texture scanning to investigate the impact of two salivary peptides, statherin (STN) and histatin-1 (HTN) 21 -terminal peptides (STN21 and HTN21), on the crystallographic structure of dental enamel. These proteins are known to play crucial roles in dental caries progression. Three healthy incisors were randomly assigned to three groups: artificially demineralized, demineralized after HTN21 peptide pre-treatment and demineralized after STN21 peptide pre-treatment. To understand the micro-scale structure of the enamel, each specimen was scanned from the enamel surface to a depth of 250 µm using microbeam EDLD. Via the use of a white beam and a pixelated detector, where each pixel functions as a spectrometer, pole figures were obtained in a single exposure at each measurement point. The results revealed distinct orientations of hydroxyapatite crystallites and notable texture variation in the peptide-treated demineralized samples compared with the demineralized control. Specifically, the peptide-treated demineralized samples exhibited up to three orientation populations, in contrast to the demineralized control which displayed only a single orientation population. The texture index of the demineralized control (2.00 ± 0.21) was found to be lower than that of either the STN21 (2.32 ± 0.20) or the HTN21 (2.90 ± 0.46) treated samples. Hence, texture scanning with EDLD gives new insights into dental enamel crystallite orientation and links the present understanding of enamel demineralization to the underlying crystalline texture. For the first time, the feasibility of EDLD texture measurements for quantitative texture evaluation in demineralized dental enamel samples is demonstrated.
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http://dx.doi.org/10.1107/S1600576724007180 | DOI Listing |
J Dent (Shiraz)
December 2024
Dept. of Restorative Dentistry, Dental Research Center, School of Dentistry, Birjand University of Medical Sciences, Birjand, Iran.
Statement Of The Problem: One of the most important factors in the clinical longevity of composite resin restorations is proper adhesion, which is achieved using phosphoric acid. Different phosphoric acid products might affect the micro-shear bond strength of composite resin to enamel.
Purpose: The present study aimed to evaluate the micro-shear bond strength of composite resin to sound enamel using six different brands of acid-etch agents.
J Dent (Shiraz)
December 2024
Dept. Conservative Dentistry and Endodontics, St.Joseph Dental College, Duggirala, Eluru, Andra Pradesh, India.
Statement Of The Problem: Dentin bonding with etch-and-rinse adhesives involves demineralizing the 5-8µm of the surface dentin to create micro space for resin infiltration. The presence of continuous fluid movement in dentin tubules and positive pulpal pressure prevents complete water replacement by resin monomers. This results in areas of demineralized dentin, which contain collagen fibers without resin infiltration.
View Article and Find Full Text PDFCommun Mater
December 2024
Department of Materials Science and Engineering, University of Washington, Seattle, WA USA.
Dental enamel is subjected to a lifetime of de- and re-mineralization cycles in the oral environment, the cumulative effects of which cause embrittlement with age. However, the understanding of atomic scale mechanisms of dental enamel aging is still at its infancy, particularly regarding where compositional differences occur in the hydroxyapatite nanocrystals and what underlying mechanisms might be responsible. Here, we use atom probe tomography to compare enamel from a young (22 years old) and a senior (56 years old) adult donor tooth.
View Article and Find Full Text PDFBMC Oral Health
December 2024
Department of Orthodontics, Faculty of Dentistry, Erciyes University, Kayseri, Melikgazi, 38039, Türkiye.
Background: The clinical performance of dental materials can be predicted via artificial aging approaches. The bracket bonding procedure may cause irreversible alterations to the enamel surface. The aim of this study was to introduce a novel artificial aging protocol and evaluate its effect on the discoloration of both bracket-bonded and unbonded teeth while also comparing the effects of four orthodontic adhesives on enamel color and whiteness.
View Article and Find Full Text PDFBMC Oral Health
December 2024
School of Stomatology, Capital Medical University, Beijing, 100050, China.
Objective: This prospective clinical study examined the effects of fixed orthodontic appliances on oral hygiene and assessed changes in the oral microflora structure of orthodontic patients using high-throughput sequencing technology.
Methods: A total of 45 patients undergoing initial fixed orthodontic treatment were recruited from three hospitals in Beijing, China. Clinical parameters and oral hygiene habits questionnaire were recorded at pre-treatment (T0) and at a 6-month follow-up (T1).
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