Introduction: Low temperature inhibits the growth of most microorganisms. However, some microbes can grow well in a low temperature, even a freezing temperature.
Methods: In this study, the mechanisms conferring cold resistance in the cryophylactic yeast () MS612, an isolate of the epidermis of ice grapes, were investigated based on comparative transcriptome analysis.
Results: A total of 6018 genes and 374 differentially expressed genes (> 2-fold, < 0.05) were identified using RNA-Seq. The differentially expressed genes were mainly involved in carbohydrate and energy metabolism, transport mechanisms, antifreeze protection, lipid synthesis, and signal transduction. MS612 maintained normal growth at low temperature (5°C) by enhancing energy metabolism, sterol synthesis, metal ion homeostasis, amino acid and MDR transport, while increased synthesis of glycerol and proline transport to improve its resistance to the freezing temperature (-5°C). Furthermore, cAMP-PKA and ERAD signaling pathways contribute to resist the low temperature and the freezing temperature, respectively.
Conclusion: This study provides new insights into cold resistance in cryophylactic microorganisms for maneuvering various metabolism to resist different cold environment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462854 | PMC |
| http://dx.doi.org/10.3389/fmicb.2024.1476087 | DOI Listing |
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