In order to interfere specifically with either the host of phage DNA metabolism and separate the effects of new phage DNA synthesis from the effects of host cell breakdown and PIL-DNA formation, UV irradiation of either the host, A. nidulans, or intact phage AS-1, prior to infection was utilized. Several conclusions were reached. First, a photoreactivation system was present in UV-irradiated A. nidulans. Second, the complete burst size of AS-1 was severely affected by UV irradiation of cells and/or phage; third, UV treatment of cells infected with healthy phage caused an early release of phage at 12 h instead of 16 h post-infection; however, healthy cells infected with UV-irradiated phage caused a delayed release of phage as 20 h.
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