SufU, a component of the SufCDSUB Fe-S cluster biosynthetic system, serves as a Zn-dependent sulfur-carrying protein that delivers inorganic sulfur in the form of cysteine persulfide from SufS to SufBCD. To understand this sulfur delivery mechanism, we studied the X-ray crystal structure of SufU and its sulfur-carrying state (persulfurated SufU) and performed functional analysis of the conserved amino acid residues around the Zn sites. Interestingly, sulfur-carrying SufU with Cys41-persulfide (Cys41-S-S) exhibited a unique Zn coordination structure, in which electrophilic S is ligated to Zn and nucleophilic/anionic S is bound to distally conserved Arg125. This structure is distinct from those of other Cys-persulfide-S-ligated metals of metalloproteins, such as hybrid cluster proteins and SoxAX. Functional analysis of SufU variants with Zn-ligand and Arg125 substitutions revealed that both Zn and Arg125 are critical for the function of SufU with SufS. The Zn-persulfide structure of SufU provides insight into the sulfur-transfer process, suggesting that persulfide-S is stabilized via bridging by Zn and Arg125 of SufU.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.inorgchem.4c02654 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stretchable and self-healing carboxymethyl cellulose/polyacrylic acid conductive hydrogels for monitoring human motions and electrophysiological signals.
Int J Biol Macromol
December 2024
State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, Xinjiang, PR China. Electronic address:
Stretchable conductive hydrogels have attracted great attention in flexible electronics. Nevertheless, conductive hydrogels would suffer from an inevitable damage during use, significantly reducing the reliability and limiting the practicability. Herein, stretchable and self-healing conductive hydrogels are designed form carboxymethyl cellulose (CMC), polyacrylic acid (PAA), and Fe, which are applied for monitoring human motions and electrophysiological signals.
View Article and Find Full Text PDFGenetic variants associated with sepsis-associated acute kidney injury.
PLoS One
December 2024
Department of Anesthesiology, Michigan Medicine, Ann Arbor, Michigan, United States of America.
Background: Kidney dysfunction is a common complication in septic patients. Studies have identified numerous risk factors for sepsis-associated acute kidney injury (S-AKI), yet there is wide variability in the incidence even among patients with similar risk factors, suggesting the presence of additional uncharacterized risk factors, including genetic differences. The expansion of biobanks, advances in genotyping, and standardized diagnostic criteria have enabled large-scale, hypothesis-generating studies into the genetic mechanisms underlying S-AKI.
View Article and Find Full Text PDFThe reduced interaction between SufS and SufU in Mycoplasma penetrans results in diminished sulfotransferase activity.
Int J Biol Macromol
January 2025
Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Key Laboratory of Energy Conversion and Storage Materials of Shanxi Provence, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:
Mycoplasma Penetrans (Mpe) is an AIDS-related mycoplasma that is also closely related to respiratory diseases. Proteins involved in the first phase of Fe-S cluster biosynthesis in the SUF-like pathway are essential in Gram-positive bacteria because there is no redundant Fe-S cluster biosynthetic pathway in these proteins. In this study, we characterized two essential proteins: cysteine desulphurase (MpeSufS) and sulfurtransferase (MpeSufU) in Mpe, and resolved their crystal structures.
View Article and Find Full Text PDFMining differentially expressed genes during paratomy in the transcriptome of the flatworm Stenostomum leucops.
Sci Rep
November 2024
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Av. Roraima 1000, Santa Maria, CEP 97105-900, RS, Brazil.
Paratomy is an asexual reproductive process that occurs in annelids, flatworms and other groups. During this process, anterior structures are formed in the middle of the body, giving rise to segmented organisms, each segment being called a zooid. Once formed, the zooids detach and form new organisms.
View Article and Find Full Text PDFAnalysis of Germline and Somatic Mutation in Patients With Developmental Odontogenic Cysts Using Targeted Gene Panel.
J Oral Pathol Med
November 2024
Department of Biochemistry, Tokyo Dental College, Chiyoda-ku, Tokyo, Japan.
Background: Odontogenic keratocyst (OKC) is a partial manifestation of Gorlin syndrome (GS), resulting from the abnormal activation of the hedgehog signaling pathway. OKC predominantly occurs in young adults and is mostly asymptomatic at the time of initial diagnosis. As OKC is asymptomatic, GS can be challenging to diagnose in certain instances.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!