AI Article Synopsis
- - The euphorbia leaf curl virus (EuLCV), a type of monopartite begomovirus, has been found to encode an additional protein called C5, alongside the usual six proteins typically found in these viruses.
- - Studies showed that the C5 protein enhances the pathogenicity and viral accumulation of potato virus X (PVX) in the plant species Nicotiana benthamiana, and it was found to localize in the cytoplasm and nucleus of cells.
- - A mutant strain of EuLCV lacking the C5 protein (EuLCV-ΔC5) demonstrated significantly lower pathogenicity and viral levels compared to the normal EuLCV, indicating that C5 plays a crucial role in the virus's
Article Abstract
The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-ΔC5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
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| http://dx.doi.org/10.1016/j.virol.2024.110252 | DOI Listing |
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