AI Article Synopsis
- Accurate tumor diagnosis is critical for effective treatment, and microRNAs (miRNAs) serve as important biomarkers in this process.
- The study introduces a colorimetric assay using ruthenium nanoparticle decorated titanium dioxide nanoribbons that allows for the on-site visualization of tumor-associated miRNAs.
- This method is highly sensitive and specific, capable of detecting miRNAs in diluted human serum with a limit of 100 picomolar, making it a promising tool for early cancer diagnosis and research.
Article Abstract
Accurate diagnosis of highly aggressive and deadly tumors is essential for effective treatment and improved patient outcomes, and microRNAs (miRNAs) have emerged as crucial biomarkers for their roles in tumor initiation, progression, and metastasis. Herein, we present an on-site visualization colorimetric assay for tumor-associated miRNAs using ruthenium nanoparticle decorated titanium dioxide nanoribbon (Ru@TiO) as a peroxidase-like (POD) nanozyme. Remarkably, the Ru@TiO nanozyme can catalyze the oxidation of chromogenic substrates through its POD-like activity, which is effectively inhibited by pyrophosphate generated during the rolling circle amplification process, thereby enabling miRNA detection through a visible colorimetric readout. This approach provides a highly sensitive and specificity assay for miRNAs in diluted human serum with a detection limit of 100 pM. It shows great potential for clinical diagnostics and biological research, offering a promising tool for early cancer diagnosis and molecular diagnostics.
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| http://dx.doi.org/10.1021/acs.analchem.4c03922 | DOI Listing |
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