Role of vitamin E on bovine skeletal-muscle-derived cells from Korean native cattle under heat treatment.

J Anim Sci

Department of Animal Science and Technology, Sanghuh College of Life Science, Konkuk University, Seoul, Korea.

Published: January 2024

AI Article Synopsis

  • The study researched how different temperatures affect muscle differentiation in bovine muscle cells, particularly looking at the effects of heat stress levels (mild vs. severe) and the protective role of vitamin E as an antioxidant.
  • Results indicated that mild heat stress (39°C) promoted early muscle differentiation, while severe heat stress (41°C) had detrimental effects on muscle-specific gene expression by day 6.
  • Vitamin E treatment showed potential in mitigating some negative impacts of severe heat stress on gene expression and protein content, highlighting its role in muscle cell health under stress conditions.

Article Abstract

Our study aimed to evaluate the dualistic effect of heat stress on muscle differentiation at different temperatures, and whether vitamin E, a powerful antioxidant, could offset any negative effects, using bovine skeletal-muscle-derived cells (BSMCs) with myogenic properties. The BSMCs were extracted from the skeletal muscle of 30-mo-old Korean native cattle and subjected to myogenic differentiation under 3 heat exposure conditions: 37 °C (control; CON), 39 °C (mild heat stress; MHS), and 41 °C (severe heat stress; SHS) for 24 h with or without vitamin E treatment (NE or VE). After 24 h treatments, the cells were returned to 37 °C incubators and differentiated until day 6. On day 1, because of the heat exposure, the gene expression of MYOG was the highest in MHS (P = 0.047), suggesting a promotive effect of mild heat stress on myogenic differentiation, while on day 6, compared with CON and MHS, MYOD (P = 0.013) and MYOG (P = 0.029) were the lowest in SHS. Vitamin E treatment also lowered MYOG (P = 0.097), regardless of heat exposure. On day 1, HSPB1 (P = 0.001) and HSP70 (P < 0.001) were the highest in SHS, and an interaction between heat exposure and vitamin E treatment was found on day 6 (P < 0.027). BCL-2 was also the highest on day 1 in SHS (P = 0.05), and an interaction of heat exposure and vitamin E treatment was found on day 1 on BAX expression (P = 0.038). For antioxidant genes, SOD1 (P = 0.002) and GPX1 (P < 0.001) were affected by heat exposure, with the highest levels being observed in SHS, and on day 6, GPX1 was still the highest in SHS (P = 0.027). The fusion index was also affected by heat exposure, showing a decrease in SHS and an increase in MHS compared with CON (P < 0.001). Significant effects were noted from heat exposure (P < 0.001), vitamin E treatment (P < 0.001), and the interaction of heat exposure and vitamin E treatment (P = 0.002) on the protein content. Taken together, our findings provide evidence that vitamin E could ameliorate the harmful effects of heat exposure by modulating heat shock proteins and apoptosis regulators, improving the protein synthesis of BSMCs during myogenic differentiation. These results suggest that vitamin E supplementation could potentially protect muscle development in beef cattle under summer heat stress.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512075PMC
http://dx.doi.org/10.1093/jas/skae292DOI Listing

Publication Analysis

Top Keywords

heat stress
16
heat exposure
12
bovine skeletal-muscle-derived
8
skeletal-muscle-derived cells
8
korean native
8
native cattle
8
heat
8
myogenic differentiation
8
mild heat
8
vitamin treatment
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!