AI Article Synopsis
- - The study investigates how replacing cytoplasm influences browning in two cytoplasmic hybrids (L808-A2 and L808-B) compared to their parent strain (L808) by analyzing nuclear, mitochondrial mRNAs, and lncRNAs over three cultivation periods (80, 100, and 120 days).
- - Results indicate that both time and cytoplasmic source affect the expression of genes related to browning, highlighting the importance of a specific NADPH-mediated antioxidant mechanism in protecting mycelia from oxidative stress caused by light exposure.
- - The research also reveals significant gene interactions affecting the mycelia's surface characteristics, including melanin accumulation, and provides new insights into communication between the nucleus and mitochondria regarding structural
Article Abstract
To explore the reason for cytoplasmic replacement's significant effect on browning, transcriptomic data of nuclear (N) and mitochondrial (M) mRNAs and long noncoding RNAs (lncRNAs) in L808 and two cytoplasmic hybrids (cybrids) (L808-A2 and L808-B) of at three different culturing times (80, 100, and 120 days) were obtained. The results showed that the expression of N and M genes and lncRNAs changed with the culture time and cytoplasmic source. Cytoplasmic replacement significantly affected some M and N genes related to the internal mechanism and external morphological characteristics of browning. The internal browning mechanism should be the nicotinamide adenine dinucleotide phosphate (NADPH)-mediated antioxidant machinery to protect mycelia against oxidative stress induced by the generation of reactive oxygen species under light irradiation. External morphological characteristics were the changing features of brown films by melanin (an antioxidant) aggregation on the surface of the mycelia of the bag or log. Especially, some genes were related to the remodeling of the plasma membrane, extracellular enzymes of celluloses and hemicellulases, small molecules, and NADPH metabolic processes. Additionally, communication between the nucleus and mitochondria mediated by M- was reported for the first time, and it is mainly appreciated in M structural assembly, functional implementation, and cooperation with other organelles.
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| http://dx.doi.org/10.1021/acs.jafc.4c03506 | DOI Listing |
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