AI Article Synopsis
- Gene expression is influenced by epigenetics and varies based on the cellular environment, with recent advancements in spatial omics enhancing our understanding of this context.
- The introduction of DBiT-seq offers a pioneering method for spatial epigenomic profiling at the cellular level, significantly improving cell typing and identification of regulatory elements.
- The new 96-channel microfluidics chip provides more precise spatial mapping of brain cells, revealing the intricate organization of glial and neuronal cells and the regulatory mechanisms that affect development and disease.
Article Abstract
Gene expression is subject to epigenetic regulation and is dependent upon cellular context. Spatial omics tools can provide insight into cellular context; however, development has centered on spatial transcriptomics and proteomics. Deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq) was the first spatial epigenomics platform at the cellular level. Here we present a comparison of spatial epigenomic profiling on both 50-channel and 96-channel platforms. The new 96-channel microfluidics chip design greatly improved precision in cell typing and identification of regulatory elements by spatial-ATAC-seq. Spatial mapping reveals complexity of glial cell and neuronal localization within brain structures as well as cis-regulatory elements controlling cellular function. This technology streamlines spatial analysis of the epigenome and contributes a new layer of spatial omics to uncover the context dependent regulatory mechanisms underpinning development, disease, and normal cellular function.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460376 | PMC |
| http://dx.doi.org/10.1089/genbio.2023.0044 | DOI Listing |
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