AI Article Synopsis
- The study investigates the role of SLAMF8 in bone healing by analyzing its effects on inflammatory responses and osteogenesis in mouse bone marrow mesenchymal stem cells (mBMSCs).
- Researchers utilized GEO datasets and various molecular techniques to assess how SLAMF8 influences genes related to bone formation (like RUNX2 and COL1A1) and fat formation (like PPARγ).
- Results showed that knocking down SLAMF8 reduced bone formation and increased fat formation in mBMSCs, while its overexpression enhanced bone healing in a femoral fracture model.
Article Abstract
Background: The inflammatory microenvironment plays an essential role in bone healing after fracture. The signaling lymphocytic activation molecule family (SLAMF) members deeply participate in inflammatory response and make a vast difference.
Methods: We identified SLAMF8 in GEO datasets (GSE129165 and GSE176086) and co-expression analyses were performed to define the relationships between SLAMF8 and osteogenesis relative genes (RUNX2 and COL1A1). In vitro, we established SLAMF8 knockdown and overexpression mouse bone marrow mesenchymal stem cells (mBMSCs) lines. qPCR, Western blot, ALP staining, ARS staining, Oil Red O staining and Immunofluorescence analyses were performed to investigate the effect of SLAMF8 in mBMSCs osteogenesis and adipogenesis. In vivo, mice femoral fracture model was performed to explore the function of SLAMF8.
Results: SLAMF8 knockdown significantly suppressed the expression of osteogenesis relative genes (RUNX2, SP7 and COL1A1), ALP activity and mineral deposition, but increased the expression of adipogenesis relative genes (PPARγ and C/EBPα). Additionally, SLAMF8 overexpression had the opposite effects. The role SLAMF8 played in mBMSCs osteogenic and adipogenic differentiation were through S100A6 and Wnt/β-Catenin signaling pathway. Moreover, SLAMF8 overexpression mBMSCs promoted the healing of femoral fracture.
Conclusions: SLAMF8 promotes osteogenesis and inhibits adipogenesis of mBMSCs via S100A6 and Wnt/β-Catenin signaling pathway. SLAMF8 overexpression mBMSCs effectively accelerate the healing of femoral fracture in mice.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462740 | PMC |
| http://dx.doi.org/10.1186/s13287-024-03964-1 | DOI Listing |
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