Objective: To determine the replication kinetics and cytopathic effect (CPE) of feline calicivirus (FCV) in feline corneal epithelial cells (FCEC).
Animals Studied: Seven archived FCV isolates and one archived feline herpesvirus type 1 (FHV-1) isolate, previously obtained from eight domestic short hair cats.
Procedures: FCV RNA was extracted for sequencing using Illumina MiSeq, to identify three genomically diverse isolates for further testing. Following reference-based assembly, viral genomes were annotated and assessed. Superficial keratectomies were performed to isolate the corneal epithelium of cats and the cells were cultured in vitro. FCEC were infected with the three chosen FCV isolates and one FHV-1 isolate at two different multiplicity of infection ratios (MOIs, 0.1 and 0.01 PFU/cell) and virus titration was assessed at 0, 2, 6, 12, 24, and 48 h post-infection (hpi). Viral identity was confirmed by RT-qPCR.
Results: Three genomically diverse FCV isolates were chosen for further assessment in the FCEC model. All infections of FCEC with FCV led to visible CPE, characterized by epithelial cell rounding and detachment from the plate by 24 hpi, while FHV-1 led to visible CPE within 48 hpi. All three of the FCV isolates replicated effectively in FCEC at both 0.1 and 0.01 MOI, with a peak increase in titer approximately 12-24 hpi.
Conclusions: The results support the possible role of FCV as a primary pathogen of the feline ocular surface. FCV replicates in FCEC in vitro, leading to profound CPE.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/vop.13289 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Screening and Immune Efficacy Evaluation of Antigens with Protection Against Feline Calicivirus.
Vaccines (Basel)
October 2024
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Background: Feline calicivirus (FCV), a pathogen that causes upper respiratory tract diseases in felids, primarily leads to oral ulcers and various respiratory symptoms, which can be fatal in severe cases. Currently, FCV prevention and control rely primarily on vaccination; however, the existing vaccine types in China are mainly inactivated vaccines, leading to a single prevention and control method with suboptimal outcomes.
Methods And Results: This study commences with a genetic evolution analysis of Chinese FCV isolates, confirming the presence of two major genotypes, GI and GII with GI emerging as the dominant form.
Yellow grub diseases on two seasonal killifish (Cyprinodontiformes, Rivulidae): a histopathological study.
Parasitol Res
November 2024
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada (LHYEDEC), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP), La Plata, Buenos Aires, Argentina.
Article Synopsis
- Yellow grub disease, caused by the parasite Clinostomum spp., affects the muscles and organs of freshwater fish, particularly killifishes in the Neotropical region, which are already vulnerable due to their short lifespan.
- * The study investigates the inflammatory damage caused by this parasite in various organs of two killifish species, revealing severe reactions especially in the pancreas, ovaries, and testes.
- * The findings suggest that this parasitic infection could lead to low fertility or sterility in seasonal killifishes, greatly affecting their ability to reproduce before their habitats dry up.
Non-consensual Sex Among Japanese Women in the COVID-19 Pandemic: A Large-Scale Nationwide Survey-Based Study.
Cureus
October 2024
Division of Epidemiology, School of Public Health, Tohoku University Graduate School of Medicine, Sendai, JPN.
Introduction and background Non-consensual sex, including rape and sexual assault, has been a global concern and may have been influenced by the COVID-19 pandemic. However, information on this topic is limited. Therefore, our objective was to survey the incidence rate of non-consensual sex among Japanese women aged 15-79 years between April and September 2020, during the early stages of the COVID-19 pandemic in Japan.
View Article and Find Full Text PDF-Containing Vacuole within : Isolation and Proteomic Characterization.
Microorganisms
September 2024
Department of Microbiology and Parasitology, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
is a highly infectious gram-negative bacterium that causes tularemia in humans and animals. It can survive and multiply in a variety of cells, including macrophages, dendritic cells, amoebae, and arthropod-derived cells. However, the intracellular life cycle of a bacterium varies depending on the cell type.
View Article and Find Full Text PDFReplication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells.
Vet Ophthalmol
October 2024
Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
Objective: To determine the replication kinetics and cytopathic effect (CPE) of feline calicivirus (FCV) in feline corneal epithelial cells (FCEC).
Animals Studied: Seven archived FCV isolates and one archived feline herpesvirus type 1 (FHV-1) isolate, previously obtained from eight domestic short hair cats.
Procedures: FCV RNA was extracted for sequencing using Illumina MiSeq, to identify three genomically diverse isolates for further testing.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!