AI Article Synopsis
- Mechanical forces play a crucial role in regulating cellular activities, and understanding these forces is key to discovering fundamental biological mechanisms.
- Researchers developed a new microprobe that can measure biological forces in living cells at the nanonewton level, enhancing experimental precision significantly.
- Using this advanced probe, they measured the elasticity modulus of HeLa cells at 1.27 ± 0.3 kPa, paving the way for more detailed studies of 3D cellular mechanics.
Article Abstract
Mechanical forces are essential for regulating dynamic changes in cellular activities. A comprehensive understanding of these forces is imperative for unraveling fundamental mechanisms. Here, we develop a microprobe capable of facilitating the measurement of biological forces up to nanonewton levels in living cells. This probe is designed by coating the core of anatase titania particles with amorphous titania and silica shells and an upconversion nanoparticles (UCNPs) layer. Leveraging both antireflection and ion resonance effects from the shells, the optically trapped probe attains a maximum lateral optical trap stiffness of 14.24 pN μm mW, surpassing the best reported value by a factor of 3. Employing this advanced probe in a photonic force microscope, we determine the elasticity modulus of mitotic HeLa cells as 1.27 ± 0.3 kPa. Nanonewton probes offer the potential to explore 3D cellular mechanics with unparalleled precision and spatial resolution, fostering a deeper understanding of the underlying biomechanical mechanisms.
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| http://dx.doi.org/10.1021/acs.nanolett.4c03610 | DOI Listing |
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