AI Article Synopsis
- Rose Bengal (RB) is a photosensitizer used to study the production of singlet oxygen, but it tends to aggregate in polar solutions when concentrations exceed 2 μM, affecting its performance.
- The research reveals that RB transitions from a monomeric to a dimeric form as its concentration increases, with different absorbance peaks indicating this change (514 nm for dimer and 549 nm for monomer).
- To ensure accurate measurements of singlet oxygen generation, it's recommended to maintain RB concentrations at or below 1 μM, highlighting the need to consider aggregation in photochemical and medical research.
Article Abstract
Rose bengal (RB) is a widely used photosensitizer for determining quantum yields of singlet oxygen generation. While it is known to aggregate in polar environments at concentrations above 2 μM, the relationship between RB concentration and singlet oxygen photogeneration remains unclear. This study investigates the shift from monomeric to dimeric RB with increasing concentration and its impact on singlet oxygen generation in DO-based solutions and DMPC liposomes. Absorbance maxima for RB were observed at 514 nm (dimer) and 549 nm (monomer), with ionic environments influencing aggregation rates. Singlet oxygen phosphorescence showed non-linear dependency above 2 μM, indicating the effects of aggregation. Results suggest that RB concentrations should be kept at 1 μM or lower in photochemical studies to avoid aggregation-related discrepancies in singlet oxygen yield determination. These findings highlight the importance of considering RB aggregation in photochemical research and medical applications.
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| http://dx.doi.org/10.1111/php.14030 | DOI Listing |
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