AI Article Synopsis
- Glyphosate use has led to the evolution of resistant weed populations, prompting a study focused on a glyphosate-resistant population (R-JX) from China.
- The R-JX population exhibited 8.5 times the resistance compared to a susceptible population, without mutations identified in the target gene; instead, increased expression and copy number of certain genes were detected.
- Pre-treatment with a P450 inhibitor reduced glyphosate resistance, while overexpressing the target gene in rice demonstrated that gene overexpression contributes to glyphosate resistance in these weed populations.
Article Abstract
Intensive application of glyphosate has resulted in resistance evolution in many weed populations, including . This study characterized glyphosate resistance and investigated the underlying mechanisms in a glyphosate-resistant population (R-JX) of from China. The R-JX population was 8.5 times resistant to glyphosate relative to the glyphosate-susceptible population (SA). Point mutations were not observed in the target gene 5-enolypyruvyl-shikimate-3-phosphate synthase gene (). However, the expression level and copy number of were 8.8 times and 15.2 times, respectively, greater in R-JX than that in the SA population. Pre-application of the P450 inhibitor lowered the resistance level to glyphosate from 8.5 times to 3.6 times in the R-JX population. RNA-Seq and RT-qPCR revealed that the gene was consistently upregulated in R-JX and five other glyphosate-resistant populations. Rice calli and seedlings overexpressing showed glyphosate resistance. In conclusion, overexpression of the target plus collectively contributes to glyphosate resistance in these populations.
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| http://dx.doi.org/10.1021/acs.jafc.4c07765 | DOI Listing |
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